[青藤碱抗肿瘤有效部位的筛选]

[Screening of anti-tumor parts from Tripterygium hypoglaucum].

作者信息

Huang Mingqing, Jiang Dongxu, Luo Mingli, Li Shaohua, Zhang Yiping, Jiang Bin, Lai Xiaoping

机构信息

Guangzhou University of Chinese Medicine, Guangzhou, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2009 Oct;34(20):2633-6.

PMID:20069909

Abstract

OBJECTIVE

To screen the anti-tumor active parts from Tripterygium hypoglaucum by anti-tumor experimental model in vivo and in vitro.

METHOD

Ethanol extraction was separated and purified by column chromatography of ion polymeric adsorbent and macroporous adsorptive resins. MTT assay and the inhibition effect to S180 solid tumor were used to detect anti-tumor activity of each separation.

RESULT

There are anti-tumor activities in the ethanol extraction, total alkaloids, and macroporous resin absorption in vivo and vitro. Minimum IC50 of Total alkaloids was 29.90 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 25, 50, 100 mg kg(-1) were 38.10%, 50.60%, 60.71% respectively. Minimum IC50 of macroporous resin absorption was 98.56 mg L(-1), and S180 solid tumor inhibition ratio of different dose of 100, 200, 400 mg kg(-1) were 42.96%, 53.57%, 63.79% respectively. Water solubility position had no effect in vivo and vitro.

CONCLUSION

T. hypoglaucum has fine anti-tumor activity in vivo and in vitro, and total alkaloids are the main part of anti-tumor active part.

摘要

目的

通过体内外抗肿瘤实验模型筛选青藤雷公藤的抗肿瘤活性部位。

方法

采用离子交换树脂柱色谱和大孔吸附树脂柱色谱对乙醇提取物进行分离纯化。采用MTT法及对S180实体瘤的抑制作用检测各分离部位的抗肿瘤活性。

结果

乙醇提取物、总生物碱及大孔树脂吸附部位在体内外均有抗肿瘤活性。总生物碱的最小IC50为29.90mg/L，25、50、100mg/kg不同剂量对S180实体瘤的抑制率分别为38.10%、50.60%、60.71%。大孔树脂吸附部位的最小IC50为98.56mg/L，100、200、400mg/kg不同剂量对S180实体瘤的抑制率分别为42.96%、53.57%、63.79%。水溶性部位在体内外均无作用。