Lee Song Eun, Ma Wanchao, Rattigan Eileen M, Aleshin Alexey, Chen Liqun, Johnson Lynne L, D'Agati Vivette D, Schmidt Ann Marie, Barile Gaetano R
Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, New York, NY, USA.
Ultrastruct Pathol. 2010 Feb;34(1):35-41. doi: 10.3109/01913120903308583.
To assess retinal capillary basement membrane thickening (BMT) in a swine model of type 1 diabetes.
Yorkshire pigs were rendered diabetic with streptozotocin and dyslipidemic with a high fat and cholesterol diet. At 18, 26, and 32 weeks of diabetes, the retina sections within 3 disc diameters from the optic disc were examined under transmission electron microscopy to evaluate the ultrastructural features of the capillary BM. Digital morphometric analysis was performed to measure BMT.
Diabetic swine had significantly thicker retinal capillary BMs compared to controls. Pigs that sustained diabetes for longer periods or experienced severe diabetes tended to have more BMT. Those pigs that did not sustain glucose levels above 200 mg/dL did not demonstrate thicker retinal capillary BMs. Characteristic ultrastructural features of diabetic vasculopathy observed included rarefaction as an early stage of Swiss cheese cavitation, lamellation with multiplication of electron dense layers, and fibrillar materials within capillary BM.
Diabetic Yorkshire pigs develop characteristic features of an early retinal microvasculopathy fairly rapidly and may serve as a higher-order animal model for studies of type 1 diabetes.
评估1型糖尿病猪模型中的视网膜毛细血管基底膜增厚（BMT）情况。
用链脲佐菌素使约克夏猪患糖尿病，并用高脂高胆固醇饮食使其血脂异常。在糖尿病病程的18、26和32周时，对距视盘3个视盘直径范围内的视网膜切片进行透射电子显微镜检查，以评估毛细血管基底膜的超微结构特征。进行数字形态计量分析以测量BMT。
与对照组相比，糖尿病猪的视网膜毛细血管基底膜明显更厚。糖尿病病程较长或患有严重糖尿病的猪往往有更多的BMT。那些血糖水平未维持在200 mg/dL以上的猪未表现出视网膜毛细血管基底膜增厚。观察到的糖尿病血管病变的特征性超微结构特征包括作为瑞士奶酪空洞形成早期阶段的稀疏、电子致密层增多的分层以及毛细血管基底膜内的纤维状物质。
糖尿病约克夏猪相当迅速地发展出早期视网膜微血管病变的特征性表现，可作为研究1型糖尿病的高级动物模型。
