Talansky B E, Malter H E, Cohen J
Department of Gynecology and Obstetrics, New York Hospital-Cornell University Medical College, New York.
Mol Reprod Dev. 1991 Feb;28(2):183-8. doi: 10.1002/mrd.1080280212.
The tendency of mammalian sperm-egg fusion to occur at a site away from the first polar body was investigated in a homologous (mouse oocytes and mouse spermatozoa) and in a heterologous model (hamster oocytes and mouse spermatozoa). Following micromanipulation of the zona pellucida either in proximity to or opposite the first polar body, in vitro fertilization was performed and subsequent differences in sperm-egg interaction were evaluated. Since spermatozoa from random-bred mice do not readily penetrate intact zonae pellucidae in vitro, it is likely that zona penetration occurred through the artificial holes in both models. The creation of a gap in the zona pellucida opposite the first polar body was associated with levels of sperm fusion that were significantly higher than those resulting from manipulation near the first polar body. Spermatozoa were rarely found to penetrate the hole completely, and in general few spermatozoa were observed in the pervitelline space. The proximity between pronuclei following sperm penetration was correlated with the position of the incision with respect to the polar body. The findings suggest that breaching the zona pellucida for microsurgical fertilization should be performed away from the microvillus-free area of the oocyte.
在同源模型（小鼠卵母细胞和小鼠精子）和异源模型（仓鼠卵母细胞和小鼠精子）中，研究了哺乳动物精卵融合在远离第一极体的部位发生的倾向。在第一极体附近或相对的位置对透明带进行显微操作后，进行体外受精，并评估随后精卵相互作用的差异。由于随机繁殖小鼠的精子在体外不易穿透完整的透明带，所以在两种模型中，精子可能都是通过人工孔穿透透明带的。在与第一极体相对的透明带处制造一个间隙，与精子融合水平显著高于在第一极体附近操作所导致的融合水平相关。很少发现精子能完全穿透孔，并且一般在卵周隙中观察到的精子很少。精子穿透后原核之间的接近程度与切口相对于极体的位置相关。研究结果表明，用于显微外科受精的透明带穿孔操作应在远离卵母细胞无微绒毛区域的地方进行。
