精液和附睾尾中衣原体科的调查以及繁殖公牛中衣原体流产嗜衣原体的血清流行率。
Investigation of Chlamydiaceae in semen and cauda epididymidis and seroprevalence of Chlamydophila abortus in breeding bulls.
机构信息
Division of Ruminant Medicine and Veterinary Epidemiology, Department of Clinical Sciences, Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-750 07, Uppsala, Sweden.
出版信息
Acta Vet Scand. 2010 Jan 13;52(1):2. doi: 10.1186/1751-0147-52-2.
BACKGROUND
Reproductive disorders associated with chlamydial infection have been reported worldwide in cattle and there are indications of potential venereal transmission.
METHODS
Semen samples from 21 dairy bulls and cauda epididymidis tissue samples from 43 beef bulls were analysed for chlamydial agent by real-time polymerase chain reaction (PCR) including an internal amplification control (mimic). Additionally, presence of antibodies against Chlamydophila (Cp.) abortus among the bulls was investigated with the commercial Pourquier ELISA Cp. abortus serum verification kit.
RESULTS
No chlamydial agent was detected by PCR in either the semen samples or in the tissue samples. Additionally, no antibodies against Cp. abortus were detected.
CONCLUSIONS
The results suggest that Cp. abortus is very rare, or absent in Swedish bulls and thus the risk for venereal transmission of chlamydial infection through their semen is low. However, because Chlamydophila spp. infection rates seem to differ throughout the world, it is essential to clarify the relative importance of transmission of the infection through semen on cattle fertility.
背景
全世界的奶牛都有与衣原体感染相关的生殖障碍的报告，并且有证据表明存在潜在的性传播。
方法
通过实时聚合酶链反应（PCR）分析了 21 头奶牛公牛的精液样本和 43 头肉牛公牛的附睪组织样本中的衣原体剂，包括内部扩增对照（模拟物）。此外，使用商业 Pourquier ELISA Chlamydophila（Cp.）abortus 血清验证试剂盒调查了公牛中针对 Chlamydophila（Cp.）abortus 的抗体的存在。
结果
在精液样本或组织样本中均未通过 PCR 检测到衣原体剂。此外，未检测到针对 Cp. abortus 的抗体。
结论
结果表明，Cp. abortus 在瑞典公牛中非常罕见或不存在，因此通过精液传播衣原体感染的性传播风险很低。然而，由于全世界的衣原体属感染率似乎有所不同，因此必须明确通过精液传播感染对牛繁殖力的相对重要性。
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