Departamento de Farmacologia e Psicobiologia, Universidade do Estado do Rio de Janeiro, Brazil.
Blood Cells Mol Dis. 2010 Mar 15;44(3):164-8. doi: 10.1016/j.bcmd.2009.12.003. Epub 2010 Jan 13.
Anorexia nervosa (AN) is associated with high cardiovascular mortality. Nitric oxide (NO) inhibits platelet function and regulates the cardiovascular homeostasis. The aim of this study was to investigate the l-arginine-NO-GMPc and arginase pathways and oxidative stress in platelets from patients with AN. Intraplatelet l-arginine transport, NOS expression and activity, cGMP levels, platelet aggregation, arginase expression and oxidative stress were measured in adolescent patients with AN (n=11) and healthy controls (n=12). Plasma l-arginine levels were significantly reduced in AN. l-arginine transport, NOS activity and cGMP basal levels were reduced in platelets associated with unchanged platelet aggregability. The expression of NOS isoforms was not affected. TBARS production was diminished, while the activity of superoxide dismutase was elevated in AN patients. There was an overexpression of arginase II in AN. Alterations of l-arginine-NO-GMPc and arginase pathways in platelets can be early predictors of the incidence of cardiovascular disease into adult life in AN.
神经性厌食症（AN）与心血管死亡率高有关。一氧化氮（NO）抑制血小板功能并调节心血管稳态。本研究旨在研究 AN 患者血小板中的 l-精氨酸-NO-GMPc 和精氨酸酶途径以及氧化应激。测量了 11 名青少年 AN 患者和 12 名健康对照者的血小板内 l-精氨酸转运、NOS 表达和活性、cGMP 水平、血小板聚集、精氨酸酶表达和氧化应激。AN 患者的血浆 l-精氨酸水平显著降低。与血小板聚集性不变相关，血小板中的 l-精氨酸转运、NOS 活性和 cGMP 基础水平降低。NOS 同工型的表达不受影响。TBARS 生成减少，而 AN 患者中超氧化物歧化酶的活性升高。AN 中存在精氨酸酶 II 的过表达。血小板中 l-精氨酸-NO-GMPc 和精氨酸酶途径的改变可能是 AN 患者成年后发生心血管疾病的早期预测指标。
