Lewrick Felicitas, Süss Regine
Department of Pharmaceutical Technology and Biopharmacy, Albert-Ludwigs University, Freiburg, Germany.
Methods Mol Biol. 2010;605:139-45. doi: 10.1007/978-1-60327-360-2_9.
The following chapter introduces a remote loading procedure for anthracyclines focussing on the well-established drug doxorubicin. The key advantage of remote loading is that it leads to higher drug to lipid ratios and encapsulation efficiencies compared to conventional passive trapping techniques like hydration of dried lipid films with aqueous drug solutions. The method presented is appropriate to produce sterile liposomal doxorubicin formulations with a final concentration of 2 mg/mL doxorubicin, which can be applied not only in vitro but also in vivo.
以下章节介绍了一种针对蒽环类药物的远程加载程序，重点是成熟药物阿霉素。远程加载的关键优势在于，与传统的被动捕获技术（如用药物水溶液水合干燥脂质膜）相比，它能实现更高的药物与脂质比例和包封效率。所介绍的方法适用于制备最终浓度为2mg/mL阿霉素的无菌脂质体阿霉素制剂，该制剂不仅可用于体外，也可用于体内。
