纵火脂质体：制备与理化特性

Arsonoliposomes: preparation and physicochemical characterization.

作者信息

Antimisiaris Sophia G, Ioannou Panayiotis V

机构信息

Laboratory of Pharmaceutical Technology, Department of Pharmacy, University of Patras, and FORTH-Institute of Chemical Engineering, Patras, Greece.

出版信息

Methods Mol Biol. 2010;605:147-62. doi: 10.1007/978-1-60327-360-2_10.

DOI:10.1007/978-1-60327-360-2_10

PMID:20072879

Abstract

Arsonoliposomes (ARSL) which are liposomes that contain arsonolipids in their membranes have shown interesting anticancer and antiparasitic activity in vitro. Their lipid composition (the specific arsonolipids and/or phospholipids used for their preparation, and the relative amounts of each lipid type) highly influences their physicochemical properties as well as their in vivo kinetics and antiparasitic activity; however, their cytotoxicity towards cancer cells is minimally--if at all--modified. ARSL are prepared by a modification of the "one step" method followed or not by sonication (for formation of sonicated or non-sonicated ARSL, respectively). Arsonoliposomes may be composed only of arsonolipids (containing or not cholesterol) [plain ARSL], or they may contain mixtures of arsonolipids with phospholipids (with or without Chol) [mixed ARSL]. Herein, we describe in detail the preparation and physicochemical characterization of ARSL.

摘要

纵火脂质体（ARSL）是一种膜中含有纵火脂质的脂质体，已在体外显示出有趣的抗癌和抗寄生虫活性。它们的脂质组成（用于制备的特定纵火脂质和/或磷脂，以及每种脂质类型的相对含量）对其物理化学性质以及体内动力学和抗寄生虫活性有很大影响；然而，它们对癌细胞的细胞毒性即使有变化也是极小的。ARSL是通过对“一步法”进行改进制备的，之后可进行超声处理（分别用于形成经超声处理或未经超声处理的ARSL）。纵火脂质体可以仅由纵火脂质组成（含或不含胆固醇）[普通ARSL]，或者它们可以包含纵火脂质与磷脂的混合物（有或没有胆固醇）[混合ARSL]。在此，我们详细描述了ARSL的制备和物理化学表征。