Palmieri Beniamino, Sblendorio Valeriana
Department of General Surgery and Surgical Specialties, Surgical Clinic, University of Modena and Reggio Emilia, Modena, Italy.
Methods Mol Biol. 2010;594:3-17. doi: 10.1007/978-1-60761-411-1_1.
Although the healthcare field is increasingly aware of the importance of free radicals and oxidative stress, screening and monitoring has not yet become a routine test, since, dangerously, there are no symptoms of this condition. Therefore, in very few cases is oxidative stress addressed. Paradoxically, patients are often advised supplementation with antioxidants and or diets with increased antioxidant profile, which ranges from vitamins to minerals and acts against oxidative stress states; even more so, no test is advised to assess whether the patient is under attack by free radicals or has a depleted antioxidant capacity. Oxidative stress i s an imbalance between free radicals (ROS, reactive oxygen species) production and existing antioxidant capacity (AC); living organisms have a complex antioxidant power. A decrease in ROS formation is often due to an increase in antioxidant capacity, while a decrease in the AC may be associated to increased ROS values. But, this is not always apparently so. Test kits for photometric determinations that are applicable to small laboratories are increasingly available.
尽管医疗保健领域越来越意识到自由基和氧化应激的重要性，但筛查和监测尚未成为常规检查，因为危险的是，这种情况没有症状。因此，在极少数情况下才会关注氧化应激。矛盾的是，患者经常被建议补充抗氧化剂和/或食用抗氧化成分增加的饮食，这些饮食从维生素到矿物质不等，可对抗氧化应激状态；更有甚者，没有人建议进行测试来评估患者是否受到自由基攻击或抗氧化能力是否耗尽。氧化应激是自由基（ROS，活性氧）产生与现有抗氧化能力（AC）之间的失衡；生物体具有复杂的抗氧化能力。ROS形成的减少通常是由于抗氧化能力的增加，而AC的降低可能与ROS值的增加有关。但是，情况并非总是如此明显。适用于小型实验室的光度测定测试试剂盒越来越多。
