Bertuglia Silvia, Intaglietta Marcos
CNR Institute of Clinical Physiology, University of Pisa, Pisa, Italy.
Methods Mol Biol. 2010;594:19-41. doi: 10.1007/978-1-60761-411-1_2.
We expose methods for in vivo assessment of oxygen, nitric oxide (NO), and reactive oxygen species (ROS)/reactive nitrogen species (RNS), in the microcirculation during normoxia and hypoxia. We provide an example of the related mechanisms of ROS/RNS and oxygen level in the process of regulating capillary perfusion. Namely, we discuss the real time pO(2) measurements in vivo in the microvessels and tissues of the hamster cheek pouch and window chamber preparations during normoxia and hypoxia, as well as the corresponding changes in ROS/RNS in systemic blood during normoxia and hypoxia under conditions where NO availability is maximally reduced.
我们阐述了在常氧和低氧条件下，对微循环中的氧气、一氧化氮（NO）以及活性氧（ROS）/活性氮（RNS）进行体内评估的方法。我们给出了一个关于ROS/RNS和氧水平在调节毛细血管灌注过程中相关机制的示例。也就是说，我们讨论了在常氧和低氧条件下，对仓鼠颊囊和视窗小室标本的微血管及组织进行体内实时pO₂测量，以及在NO可用性最大程度降低的条件下，常氧和低氧时全身血液中ROS/RNS的相应变化。
