Terrón-Camero Laura C, Molina-Moya Eliana, Sanz-Fernández María, Sandalio Luisa M, Romero-Puertas María C
Department of Biochemistry and Cellular and Molecular Biology of Plants, Estación Experimental del Zaidín (CSIC), Granada, Spain.
Methods Mol Biol. 2018;1743:97-105. doi: 10.1007/978-1-4939-7668-3_9.
Reactive oxygen and nitrogen species (ROS/RNS) are signaling molecules involved in a plethora of physiological processes in plants. Especially, ROS and nitric oxide (NO) are key players that are required for programmed cell death (PCD). The PCD associated with the hypersensitive response (HR) has been well characterized and the role of HO and NO as key signaling molecules inducing HR has been established. Localization of ROS and NO production in plant tissues in response to pathogens can be imaged by confocal laser microscopy by using specific fluorescent probes. Deciphering the time and spatial regulation of ROS and NO is very important to establish the cellular response of plants to adverse conditions. This chapter is mainly focused on the imaging of ROS and RNS accumulation in vivo in plant tissues undergoing PCD.
活性氧和氮化物（ROS/RNS）是参与植物众多生理过程的信号分子。特别是，ROS和一氧化氮（NO）是程序性细胞死亡（PCD）所必需的关键因子。与超敏反应（HR）相关的PCD已得到充分表征，并且已确定H₂O₂和NO作为诱导HR的关键信号分子的作用。通过使用特定的荧光探针，可利用共聚焦激光显微镜对植物组织中响应病原体时ROS和NO的产生进行定位成像。解读ROS和NO的时间和空间调控对于确定植物对不利条件的细胞反应非常重要。本章主要聚焦于对经历PCD的植物组织中ROS和RNS体内积累的成像研究。
