Petrovic Nenad, Murray Michael
School of Pharmacy, Murdoch University, Murdoch, WA, Australia.
Methods Mol Biol. 2010;594:129-40. doi: 10.1007/978-1-60761-411-1_9.
Prostaglandin endoperoxide synthase (PGH synthase), also known as cyclooxygenase (COX), was identified over 30 years ago and is the key enzyme in the pathway by which arachidonic acid is converted to the range of biologically active lipid mediators known as the prostanoids that participate in numerous physiological processes. The need for the development of new and improved COX inhibitors as potential therapeutics also drives the need for rapid, reliable, and inexpensive assays of COX activity. Colorimetric assays are often the preferred methods of enzyme analysis since they may be readily adapted to simple microplate formats that require relatively inexpensive and widely available instrumentation. The use of N,N,N cent,N cent-tetramethyl-p-phenylenediamine (TMPD) in high throughput microplate assays of COX activity could become the approach of choice in the screening of potential therapeutics that inhibit COX activity in vivo. Considering that TMPD is also a potential substrate for most, if not all, heme peroxidases, it is anticipated that this agent could find increasing application in the future.
前列腺素内过氧化物合酶（PGH合酶），也称为环氧化酶（COX），是30多年前发现的，它是花生四烯酸转化为一系列称为前列腺素的生物活性脂质介质途径中的关键酶，这些前列腺素参与众多生理过程。开发新型且改良的COX抑制剂作为潜在治疗药物的需求，也推动了对快速、可靠且廉价的COX活性检测方法的需求。比色法检测通常是酶分析的首选方法，因为它们可以很容易地适用于简单的微孔板形式，这种形式需要相对便宜且广泛可用的仪器。在COX活性的高通量微孔板检测中使用N，N，N′，N′-四甲基对苯二胺（TMPD）可能会成为筛选体内抑制COX活性的潜在治疗药物的首选方法。鉴于TMPD即使不是所有血红素过氧化物酶的潜在底物，也是大多数血红素过氧化物酶的潜在底物，预计该试剂在未来会有越来越多的应用。
