Department of Infectious Parasitic and Immune-mediated Diseases, Superior Institute of Health, Rome, Italy.
Int J Immunopathol Pharmacol. 2009 Oct-Dec;22(4):967-78. doi: 10.1177/039463200902200412.
This study reports the immunomodulatory activity on human monocyte derived dendritic cells (MDDCs) of a vaccine preparation shown to be effective against an HPV16-related tumour in an animal model. The vaccine is composed of extract from Nicotiana benthamiana leaves containing HPV16 E7 protein expressed by a potato virus X-derived vector (NbPVX-E7). The effect of the extract was evaluated on MDDC differentiation and maturation by monitoring the phenotypic expression of specific markers. The results show that NbPVX-E7 does not induce monocyte differentiation to dendritic cells, but does induce MDDC maturation. Plant extract does not influence MDDC-uptake of E7-FITC while it significantly improves the Ovalbumin-FITC uptake, considered as a model antigen. Importantly, NbPVX-E7-pulsed MDDCs/PBMCs are able to prime human blood-derived lymphocytes from healthy individuals to induce HPV16 E7-specific cytotoxic activity. This is a propaedeutic study for a possible use of E7-containing plant extract in human immunotherapy of HPV-related lesions.
本研究报告了一种疫苗制剂对人单核细胞衍生树突状细胞（MDDC）的免疫调节活性，该疫苗在动物模型中已被证明对 HPV16 相关肿瘤有效。该疫苗由含有 HPV16 E7 蛋白的 Nicotiana benthamiana 叶提取物组成，由马铃薯病毒 X 衍生载体（NbPVX-E7）表达。通过监测特定标记物的表型表达来评估提取物对 MDDC 分化和成熟的影响。结果表明，NbPVX-E7 不会诱导单核细胞分化为树突状细胞，但会诱导 MDDC 成熟。植物提取物不影响 MDDC 摄取 E7-FITC，而显著提高 Ovalbumin-FITC 的摄取，被认为是一种模型抗原。重要的是，NbPVX-E7 脉冲的 MDDC/PBMC 能够从健康个体的人血液衍生淋巴细胞中诱导 HPV16 E7 特异性细胞毒性活性。这是一种可能在 HPV 相关病变的人类免疫治疗中使用含 E7 的植物提取物的初步研究。
