ND2 亚基被鱼藤酮的一种光亲和类似物标记，鱼藤酮是一种有效的复合物 I 抑制剂。
The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor.
机构信息
Department of Molecular and Experimental Medicine, The Scripps Research Institute, La Jolla, CA 92037, USA.
出版信息
FEBS Lett. 2010 Mar 5;584(5):883-8. doi: 10.1016/j.febslet.2010.01.004. Epub 2010 Jan 13.
Abstract
NADH
ubiquinone oxidoreductase (complex I) is the entry enzyme of mitochondrial oxidative phosphorylation. To obtain the structural information on inhibitor/quinone binding sites, we synthesized [3H]benzophenone-asimicin ([3H]BPA), a photoaffinity analogue of asimicin, which belongs to the acetogenin family known as the most potent complex I inhibitor. We found that [3H]BPA was photo-crosslinked to ND2, ND1 and ND5 subunits, by the three dimensional separation (blue-native/doubled SDS-PAGE) of [3H]BPA-treated bovine heart submitochondrial particles. The cross-linking was blocked by rotenone. This is the first finding that ND2 was photo-crosslinked with a potent complex I inhibitor, suggesting its involvement in the inhibitor/quinone-binding.
摘要
烟酰胺腺嘌呤二核苷酸
泛醌氧化还原酶（复合体 I）是线粒体氧化磷酸化的入口酶。为了获得抑制剂/醌结合位点的结构信息，我们合成了[3H]苯并酮-asi 霉素([3H]BPA)，这是 asi 霉素的光亲和类似物，asi 霉素属于被称为最有效的复合体 I 抑制剂的乙酰生酮家族。我们发现[3H]BPA 通过牛心亚线粒体颗粒的三维分离（蓝色非变性/双重 SDS-PAGE）与 ND2、ND1 和 ND5 亚基发生光交联。这种交联被鱼藤酮所阻断。这是首次发现 ND2 与一种强效的复合体 I 抑制剂发生光交联，表明它参与了抑制剂/醌结合。
相似文献
1
The ND2 subunit is labeled by a photoaffinity analogue of asimicin, a potent complex I inhibitor.
FEBS Lett. 2010 Mar 5;584(5):883-8. doi: 10.1016/j.febslet.2010.01.004. Epub 2010 Jan 13.
2
Exploring interactions between the 49 kDa and ND1 subunits in mitochondrial NADH-ubiquinone oxidoreductase (complex I) by photoaffinity labeling.
Biochemistry. 2011 Aug 16;50(32):6901-8. doi: 10.1021/bi200883c. Epub 2011 Jul 8.
3
Exploring the binding site of delta(lac)-acetogenin in bovine heart mitochondrial NADH-ubiquinone oxidoreductase.
Biochemistry. 2010 Jun 15;49(23):4794-803. doi: 10.1021/bi100454b.
4
The ND1 subunit constructs the inhibitor binding domain in bovine heart mitochondrial complex I.
Biochemistry. 2007 May 29;46(21):6409-16. doi: 10.1021/bi7003697. Epub 2007 May 3.
5
Fenpyroximate binds to the interface between PSST and 49 kDa subunits in mitochondrial NADH-ubiquinone oxidoreductase.
Biochemistry. 2012 Mar 6;51(9):1953-63. doi: 10.1021/bi300047h. Epub 2012 Feb 27.
6
Exploring the binding site of acetogenin in the ND1 subunit of bovine mitochondrial complex I.
Biochim Biophys Acta. 2009 Sep;1787(9):1106-11. doi: 10.1016/j.bbabio.2009.02.016. Epub 2009 Mar 2.
7
Characterization of the inhibitor binding site in mitochondrial NADH-ubiquinone oxidoreductase by photoaffinity labeling using a quinazoline-type inhibitor.
Biochemistry. 2009 Feb 3;48(4):688-98. doi: 10.1021/bi8019977.
8
Bis-THF motif of acetogenin binds to the third matrix-side loop of ND1 subunit in mitochondrial NADH-ubiquinone oxidoreductase.
Biochim Biophys Acta. 2011 Sep;1807(9):1170-6. doi: 10.1016/j.bbabio.2011.05.012. Epub 2011 May 17.
9
The ND5 subunit was labeled by a photoaffinity analogue of fenpyroximate in bovine mitochondrial complex I.
Biochemistry. 2003 Jan 28;42(3):746-54. doi: 10.1021/bi0269660.
10
Essential structural features of acetogenins: role of hydroxy groups adjacent to the bis-THF rings.
Bioorg Med Chem Lett. 2004 Feb 9;14(3):779-82. doi: 10.1016/j.bmcl.2003.11.021.
引用本文的文献
1
Identification of single nucleotide polymorphisms (SNPs) potentially associated with residual feed intake in Qinchuan beef cattle by hypothalamus and duodenum RNA-Seq data.
PeerJ. 2025 Apr 15;13:e19270. doi: 10.7717/peerj.19270. eCollection 2025.
2
Characterization of the Complete Mitochondrial Genome of from the South China Sea.
Int J Mol Sci. 2023 Dec 26;25(1):357. doi: 10.3390/ijms25010357.
3
Monkfish Peptides Mitigate High Fat Diet-Induced Hepatic Steatosis in Mice.
Mar Drugs. 2022 May 5;20(5):312. doi: 10.3390/md20050312.
4
Comparative transcriptome analysis in the hepatopancreas of Helice tientsinensis exposed to the toxic metal cadmium.
Genes Genomics. 2019 Apr;41(4):417-429. doi: 10.1007/s13258-018-0774-1. Epub 2018 Dec 7.
5
Transcriptome assembly and expression profiling of the molecular responses to cadmium toxicity in cerebral ganglia of wolf spider Pardosa pseudoannulata (Araneae: Lycosidae).
Ecotoxicology. 2018 Mar;27(2):198-208. doi: 10.1007/s10646-017-1885-1. Epub 2018 Jan 3.
6
A Novel Positron Emission Tomography (PET) Approach to Monitor Cardiac Metabolic Pathway Remodeling in Response to Sunitinib Malate.
PLoS One. 2017 Jan 27;12(1):e0169964. doi: 10.1371/journal.pone.0169964. eCollection 2017.
7
Gene expression profiling analysis of lung adenocarcinoma.
Braz J Med Biol Res. 2016 Mar;49(3). doi: 10.1590/1414-431X20154861. Epub 2016 Feb 2.
8
Role of Drug Metabolism in the Cytotoxicity and Clinical Efficacy of Anthracyclines.
Curr Drug Metab. 2015;16(6):412-26. doi: 10.2174/1389200216888150915112039.
9
Semiquinone intermediates are involved in the energy coupling mechanism of E. coli complex I.
Biochim Biophys Acta. 2015 Aug;1847(8):681-9. doi: 10.1016/j.bbabio.2015.04.004. Epub 2015 Apr 11.
10
Physiological and comparative proteomic analysis reveals different drought responses in roots and leaves of drought-tolerant wild wheat (Triticum boeoticum).
PLoS One. 2015 Apr 10;10(4):e0121852. doi: 10.1371/journal.pone.0121852. eCollection 2015.
本文引用的文献
1
Exploring the binding site of acetogenin in the ND1 subunit of bovine mitochondrial complex I.
Biochim Biophys Acta. 2009 Sep;1787(9):1106-11. doi: 10.1016/j.bbabio.2009.02.016. Epub 2009 Mar 2.
2
Characterization of the inhibitor binding site in mitochondrial NADH-ubiquinone oxidoreductase by photoaffinity labeling using a quinazoline-type inhibitor.
Biochemistry. 2009 Feb 3;48(4):688-98. doi: 10.1021/bi8019977.
3
Binding is not enough: flexibility is needed for photocrosslinking of Lck kinase by benzophenone photoligands.
Bioorg Med Chem. 2008 Oct 1;16(19):8824-9. doi: 10.1016/j.bmc.2008.08.077. Epub 2008 Sep 3.
4
Identification of the mitochondrial ND3 subunit as a structural component involved in the active/deactive enzyme transition of respiratory complex I.
J Biol Chem. 2008 Jul 25;283(30):20907-13. doi: 10.1074/jbc.M803190200. Epub 2008 May 23.
5
The ND1 subunit constructs the inhibitor binding domain in bovine heart mitochondrial complex I.
Biochemistry. 2007 May 29;46(21):6409-16. doi: 10.1021/bi7003697. Epub 2007 May 3.
6
Blue native PAGE.
Nat Protoc. 2006;1(1):418-28. doi: 10.1038/nprot.2006.62.
7
Tricine-SDS-PAGE.
Nat Protoc. 2006;1(1):16-22. doi: 10.1038/nprot.2006.4.
8
Analysis of the synaptic vesicle proteome using three gel-based protein separation techniques.
Proteomics. 2006 Dec;6(23):6250-62. doi: 10.1002/pmic.200600357.
9
Bovine complex I is a complex of 45 different subunits.
J Biol Chem. 2006 Oct 27;281(43):32724-7. doi: 10.1074/jbc.M607135200. Epub 2006 Sep 1.
10
Energy converting NADH:quinone oxidoreductase (complex I).
Annu Rev Biochem. 2006;75:69-92. doi: 10.1146/annurev.biochem.75.103004.142539.
Zotero 插件