Stone Judy
Clinical Laboratories, San Francisco General Hospital, San Francisco, CA 94804, USA.
Methods Mol Biol. 2010;603:203-18. doi: 10.1007/978-1-60761-459-3_19.
Centrifuged urine, internal standard (promazine), and ammonium formate buffer are mixed in an autosampler vial to achieve a 10-fold dilution of the specimen. Without additional pretreatment, 10 microL of the sample is injected onto a C18 reverse phase column for gradient analysis with ammonium formate/acetonitrile mobile phases. Drugs in the column eluent become charged in the ion source using positive electrospray atmospheric pressure ionization. Pseudomolecular drug ions are analyzed by a hybrid triple quadrupole linear ion trap mass spectrometer operated with a 264-drug selected ion monitoring (SRM) acquisition method that includes an information-dependant acquisition (IDA) algorithm.
将离心后的尿液、内标物（丙嗪）和甲酸铵缓冲液在自动进样器小瓶中混合，使样品稀释10倍。无需额外预处理，将10微升样品注入C18反相柱，用甲酸铵/乙腈流动相进行梯度分析。柱洗脱液中的药物在离子源中使用正电喷雾大气压电离法带电。通过采用264种药物选择离子监测（SRM）采集方法（包括信息依赖采集（IDA）算法）运行的混合三重四极杆线性离子阱质谱仪分析假分子药物离子。
