Department of Forestry, National Chung Hsing University, Core Laboratory of Plant Metabolomics in Biotechnology Centre, Taichung 402, Taiwan.
Phytother Res. 2010 Aug;24(8):1158-65. doi: 10.1002/ptr.3018.
The effects of lucidone on tyrosinase and antimelanogenic activity were investigated. Initially, we found that lucidone strongly inhibits the activity of mushroom tyrosinase. The effects of lucidone on tyrosinase were further examined in alpha-MSH-induced B16 melanoma cells. Lucidone significantly inhibits tyrosinase activity and leads to decreased melanin content in cultured B16 melanoma cells. Lucidone also attenuates the expression of tyrosinase and MITF (Microphthalmia-associated Transcription Factor) protein in a concentration-dependent manner, as shown by western blot. Quantitative real-time reverse-transcription polymerase chain reaction (RT-PCR) confirmed that lucidone inhibits the expression of tyrosinase mRNA. Accordingly, the effects of lucidone on the ERK signaling pathway were also investigated, but lucidone was not found to play major role in the induction of ERK activation. Our data indicate that the antimelanogenic activity of lucidone is probably due to its inhibition of tyrosinase activity and the suppression of tyrosinase and MITF expression.
我们研究了露西酮对酪氨酸酶和抗黑色素生成活性的影响。最初，我们发现露西酮强烈抑制蘑菇酪氨酸酶的活性。在α-MSH 诱导的 B16 黑色素瘤细胞中进一步研究了露西酮对酪氨酸酶的影响。露西酮显著抑制酪氨酸酶活性，并导致培养的 B16 黑色素瘤细胞中黑色素含量降低。Western blot 显示，露西酮还呈浓度依赖性地减弱酪氨酸酶和 MITF（小眼畸形相关转录因子）蛋白的表达。实时定量逆转录聚合酶链反应 (RT-PCR) 证实露西酮抑制酪氨酸酶 mRNA 的表达。因此，还研究了露西酮对 ERK 信号通路的影响，但未发现露西酮在诱导 ERK 激活中起主要作用。我们的数据表明，露西酮的抗黑色素生成活性可能归因于其对酪氨酸酶活性的抑制以及对酪氨酸酶和 MITF 表达的抑制。
Zotero 插件