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木材精油的抗黑色素生成活性及其化学成分分析

Anti-Melanogenic Activity of Wood Essential Oil and Its Chemical Composition Analysis.

作者信息

Hsiao Wen-Wei, Kumar K J Senthil, Lee Hui-Ju, Tsao Nai-Wen, Wang Sheng-Yang

机构信息

Experimental Forest, College of Bio-Resources and Agriculture, National Taiwan University, Taipei 10617, Taiwan.

Bachelor Program of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan.

出版信息

Plants (Basel). 2021 Dec 25;11(1):62. doi: 10.3390/plants11010062.

Abstract

(Cupressaceae) is one of the five precious woods of Taiwan. In this study, we investigated the anti-melanogenic activity of wood essential oil (CFEO) and its bioactive components in vitro. Initially, CFEO exhibited strong mushroom tyrosinase activity in the cell-free mushroom tyrosinase assay system with an IC value of 2.72 µg/mL. Next, treatment with CFEO significantly as well as dose-dependently reduced a combination of α-melanocyte-stimulating hormone and forskolin (α-MSH-FSK)-induced melanin synthesis in B16-F10 cells. Indeed, 80 μg/mL CFEO completely inhibited melanin production, which is similar to that of control cells. Further studies revealed that treatment with CFEO significantly inhibited melanogenesis regulatory proteins, including TRP-1, TRP-2, and MITF, whereas tyrosinase was unaffected by either α-MSH-FSK or CFEO. In addition, the composition of the CFEO was characterized. The major components of CFEO were α-terpineol (23.47%), shonanic acid (10.45%), terpinen-4-ol (12.23%), thymol (5.3%), piperitone (3.44%), berbenone (2.81%), thujic acid (1.65%), and chaminic acid (0.13%). Among them, shonanic acid (), thujic acid (), and chaminic acid () were uncommon constitutes in essential oils, which could be the index compounds of CFEO, and the structure of these compounds were confirmed by spectral analysis. Furthermore, we found that thymol is an active ingredient responsible for CFEO's anti-melanogenic activity. Based on these results, we suggest that CFEO or thymol could be a potential candidate for the development of skin whitening products for cosmetic purposes.

摘要

柏科植物是台湾的五种珍贵木材之一。在本研究中,我们体外研究了柏木精油(CFEO)及其生物活性成分的抗黑色素生成活性。最初,在无细胞蘑菇酪氨酸酶测定系统中,CFEO表现出较强的蘑菇酪氨酸酶活性,IC值为2.72μg/mL。接下来,用CFEO处理显著且剂量依赖性地降低了α-黑素细胞刺激素和福斯可林(α-MSH-FSK)诱导的B16-F10细胞中的黑色素合成。实际上,80μg/mL的CFEO完全抑制了黑色素的产生,这与对照细胞相似。进一步的研究表明,用CFEO处理显著抑制了黑色素生成调节蛋白,包括TRP-1、TRP-2和MITF,而酪氨酸酶不受α-MSH-FSK或CFEO的影响。此外,对CFEO的成分进行了表征。CFEO的主要成分是α-松油醇(23.47%)、肖楠酸(10.45%)、萜品-4-醇(12.23%)、百里香酚(5.3%)、哌啶酮(3.44%)、柏木酮(2.81%)、土荆酸(1.65%)和查米酸(0.13%)。其中,肖楠酸、土荆酸和查米酸是精油中不常见的成分,可能是CFEO的指标化合物,这些化合物的结构通过光谱分析得到证实。此外,我们发现百里香酚是负责CFEO抗黑色素生成活性的活性成分。基于这些结果,我们认为CFEO或百里香酚可能是开发用于化妆品目的的美白产品的潜在候选物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/67ad/8747218/518303353bd6/plants-11-00062-g001.jpg

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