National Center for Chronic Disease Prevention and Health Promotion, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, USA.
BMC Med Genet. 2010 Jan 15;11:6. doi: 10.1186/1471-2350-11-6.
Evidence on the associations of emerging cardiovascular disease risk factors/markers with genes may help identify intermediate pathways of disease susceptibility in the general population. This population-based study is aimed to determine the presence of associations between a wide array of genetic variants and emerging cardiovascular risk markers among adult US women.
The current analysis was performed among the National Health and Nutrition Examination Survey (NHANES) III phase 2 samples of adult women aged 17 years and older (sample size n = 3409). Fourteen candidate genes within ADRB2, ADRB3, CAT, CRP, F2, F5, FGB, ITGB3, MTHFR, NOS3, PON1, PPARG, TLR4, and TNF were examined for associations with emerging cardiovascular risk markers such as serum C-reactive protein, homocysteine, uric acid, and plasma fibrinogen. Linear regression models were performed using SAS-callable SUDAAN 9.0. The covariates included age, race/ethnicity, education, menopausal status, female hormone use, aspirin use, and lifestyle factors.
In covariate-adjusted models, serum C-reactive protein concentrations were significantly (P value controlling for false-discovery rate < or = 0.05) associated with polymorphisms in CRP (rs3093058, rs1205), MTHFR (rs1801131), and ADRB3 (rs4994). Serum homocysteine levels were significantly associated with MTHFR (rs1801133).
The significant associations between certain gene variants with concentration variations in serum C-reactive protein and homocysteine among adult women need to be confirmed in further genetic association studies.
新兴心血管疾病风险因素/标志物与基因之间关联的证据可能有助于确定一般人群中疾病易感性的中间途径。本研究旨在确定美国成年女性中广泛的遗传变异与新兴心血管风险标志物之间是否存在关联。
目前的分析是在国家健康和营养调查（NHANES）III 期 2 阶段的成年女性样本中进行的，年龄在 17 岁及以上（样本量 n = 3409）。在 ADRB2、ADRB3、CAT、CRP、F2、F5、FGB、ITGB3、MTHFR、NOS3、PON1、PPARG、TLR4 和 TNF 中，有 14 个候选基因与新兴心血管风险标志物（如血清 C 反应蛋白、同型半胱氨酸、尿酸和血浆纤维蛋白原）进行了关联分析。使用 SAS 可调用的 SUDAAN 9.0 进行线性回归模型分析。协变量包括年龄、种族/民族、教育程度、绝经状态、女性激素使用、阿司匹林使用和生活方式因素。
在调整协变量的模型中，血清 C 反应蛋白浓度与 CRP(rs3093058、rs1205)、MTHFR(rs1801131)和 ADRB3(rs4994)的多态性显著相关（P 值控制假发现率<或=0.05）。血清同型半胱氨酸水平与 MTHFR(rs1801133)显著相关。
在成年女性中，某些基因变异与血清 C 反应蛋白和同型半胱氨酸浓度变化之间存在显著关联，需要在进一步的遗传关联研究中得到证实。
