[应用T细胞淋巴瘤抗体1和CD44进行免疫组织化学研究在伯基特淋巴瘤诊断中的应用]

[Immunohistochemical study using T-cell lymphoma antibody 1 and CD44 in diagnosis of Burkitt's lymphoma].

作者信息

Shui Ruo-hong, Lu Hong-fen, Zhu Xiong-zeng

机构信息

Department of Pathology, Cancer Hospital, Fudan University, Shanghai 200032, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2009 Nov;38(11):745-8.

PMID:20079013

Abstract

OBJECTIVE

To study the values of immunohistochemistry using T-cell lymphoma antibody (TCL) 1 and CD44 in the diagnosis of Burkitt's lymphoma.

METHODS

Immunohistochemical study for TCL1, CD44, CD10, bcl-2, bcl-6, c-myc and Ki-67 was performed on paraffin-embedded sections of lymphoma cases, including 25 cases of Burkitt's lymphoma and 25 cases of diffuse large B-cell lymphoma.

RESULTS

Burkitt's lymphoma commonly expressed TCL1 (96%, 24 cases), CD10 (88%, 22 cases), bcl-6 and c-myc (92%, 23 cases). Only 1 case (4%) expressed CD44 and bcl-2. The Ki-67 proliferation index ranged from 95% to 100%. On the other hand, diffuse large B-cell lymphoma expressed CD44 (84%, 21 cases), CD10 (32%, 8 cases), bcl-6 (72%, 18 cases) and bcl-2 (72%, 18 cases). Four cases (16%) were weakly positive for TCL1. The staining for c-myc was all negative. The Ki-67 proliferation index ranged from 40% to 90%.

CONCLUSION

Immunohistochemical staining for TCL1 and CD44 is a useful ancillary tool in the pathologic diagnosis of Burkitt's lymphoma which is also helpful for the differential diagnosis from diffuse large B-cell lymphoma.

摘要

目的

研究使用T细胞淋巴瘤抗体（TCL）1和CD44进行免疫组化在伯基特淋巴瘤诊断中的价值。

方法

对淋巴瘤病例的石蜡包埋切片进行TCL1、CD44、CD10、bcl-2、bcl-6、c-myc和Ki-67的免疫组化研究，其中包括25例伯基特淋巴瘤和25例弥漫性大B细胞淋巴瘤。

结果

伯基特淋巴瘤通常表达TCL1（96%，24例）、CD10（88%，22例）、bcl-6和c-myc（92%，23例）。仅1例（4%）表达CD44和bcl-2。Ki-67增殖指数范围为95%至100%。另一方面，弥漫性大B细胞淋巴瘤表达CD44（84%，21例）、CD10（32%，8例）、bcl-6（72%，18例）和bcl-2（72%，18例）。4例（16%）TCL1弱阳性。c-myc染色均为阴性。Ki-67增殖指数范围为40%至90%。