Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstrasse 108, D-01307 Dresden, Germany.
Curr Biol. 2010 Jan 26;20(2):161-9. doi: 10.1016/j.cub.2009.11.036. Epub 2010 Jan 14.
Collective cell migration, the simultaneous movement of multiple cells that are connected by cell-cell adhesion, is ubiquitous in development, tissue repair, and tumor metastasis [1, 2]. It has been hypothesized that the directionality of cell movement during collective migration emerges as a collective property [3, 4]. Here we determine how movement directionality is established in collective mesendoderm migration during zebrafish gastrulation. By interfering with two key features of collective migration, (1) having neighboring cells and (2) adhering to them, we show that individual mesendoderm cells are capable of normal directed migration when moving as single cells but require cell-cell adhesion to participate in coordinated and directed migration when moving as part of a group. We conclude that movement directionality is not a de novo collective property of mesendoderm cells but rather a property of single mesendoderm cells that requires cell-cell adhesion during collective migration.
细胞集体迁移是指多个通过细胞间黏附连接在一起的细胞同时运动，这种现象在发育、组织修复和肿瘤转移中普遍存在[1,2]。人们假设细胞在集体迁移过程中的运动方向是作为一个集体属性出现的[3,4]。在这里，我们确定了在斑马鱼原肠胚形成过程中，中胚层集体迁移中运动方向是如何建立的。通过干扰集体迁移的两个关键特征，（1）有相邻的细胞和（2）与它们黏附，我们表明，当单个中胚层细胞作为单细胞移动时，它们能够进行正常的定向迁移，但当作为一个群体的一部分移动时，它们需要细胞间黏附才能参与协调和定向迁移。我们的结论是，运动方向不是中胚层细胞的新的集体属性，而是单个中胚层细胞的属性，在集体迁移过程中需要细胞间黏附。
