Department of Nutrition, University of Oslo, Blindern, N-0316 Oslo, Norway.
Biochem Biophys Res Commun. 2010 Feb 12;392(3):436-41. doi: 10.1016/j.bbrc.2010.01.042. Epub 2010 Jan 15.
The present study addresses the insulin sensitivity in mice deficient in LXRbeta (LXRbeta(-/-)) as well as in wild type (wt) mice assessed by hyperinsulinemic euglycemic clamp. Wt and LXRbeta(-/-) mice were fed either a normal chow diet or a high fat and high cholesterol diet (HFCD), and insulin sensitivity was assessed by hyperinsulinemic euglycemic clamps. We show that LXRbeta(-/-) mice have reduced insulin clearance during hyperinsulinemic clamps upon feeding both HFCD and a regular chow diet. Moreover we also observed reduced hepatic inflammation in LXRbeta(-/-) mice compared to wt mice upon feeding an HFCD, despite equal levels of hepatic steatosis. In summary, our results indicate that LXRbeta(-/-) mice have reduced insulin clearance during hyperinsulinemic euglycemic clamps and also reduced hepatic inflammation upon feeding an HFCD for 26weeks.
本研究探讨了 LXRβ 缺陷（LXRβ（-/-））小鼠以及通过高胰岛素-正常血糖钳夹评估的野生型（wt）小鼠的胰岛素敏感性。wt 和 LXRβ（-/-）小鼠分别喂食正常饲料或高脂肪高胆固醇饮食（HFCD），并通过高胰岛素-正常血糖钳夹评估胰岛素敏感性。我们发现，在喂食 HFCD 和常规饲料时，LXRβ（-/-）小鼠在高胰岛素钳夹期间的胰岛素清除率降低。此外，我们还观察到，尽管肝脂肪变性水平相等，但在喂食 HFCD 时，LXRβ（-/-）小鼠的肝炎症也减少。总之，我们的结果表明，LXRβ（-/-）小鼠在高胰岛素-正常血糖钳夹期间的胰岛素清除率降低，并且在喂食 HFCD 26 周后肝炎症也减少。
