College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Republic of Korea.
Fitoterapia. 2010 Sep;81(6):497-502. doi: 10.1016/j.fitote.2010.01.003. Epub 2010 Jan 18.
The effects of scoparone on dopamine release in PC12 cells were investigated. Scoparone at 50-200 microM increased dopamine release into the culture medium. However, the released levels of dopamine by scoparone were not altered in the absence of extracellular Ca(2+) and by adenylyl cyclase inhibitor MDL-12,330A. Scoparone increased phosphorylation of PKA, CaMK II and synapsin I. Scoparone also enhanced K(+)-induced levels of dopamine release by CaMK II phosphorylation. These results suggest that scoparone increases dopamine release by synapsin I phosphorylation via activation of PKA and CaMK II, which are mediated by cyclic AMP levels and Ca(2+) influx.
研究了莪术酮对 PC12 细胞多巴胺释放的影响。50-200μM 的莪术酮增加了多巴胺向培养基中的释放。然而，在不存在细胞外 Ca(2+)和腺苷酸环化酶抑制剂 MDL-12,330A 的情况下，莪术酮对多巴胺释放的水平没有改变。莪术酮增加了 PKA、CaMK II 和突触蛋白 I 的磷酸化。莪术酮还通过 CaMK II 磷酸化增强了 K(+)诱导的多巴胺释放。这些结果表明，莪术酮通过激活 PKA 和 CaMK II 使突触蛋白 I 磷酸化，从而增加多巴胺释放，这是由环 AMP 水平和 Ca(2+)内流介导的。
