Department of Vascular Surgery, First Affiliated Hospital of Chongqing Medical University, Chongqing, China.
J Surg Res. 2011 Jul;169(1):148-55. doi: 10.1016/j.jss.2009.09.029. Epub 2009 Oct 6.
The long-term patency rates in vascular transplants (diameter<3.0-4.0mm) are very low due to thrombus formation and intimal hyperplasia. A possible mechanism is the loss of the endothelial cells (ECs) lining. Previous attempts to reseed ECs had poor results due to seeded cell loss, severe antigenicity, and low compliance. The objectives of this study were to generate an allogeneic vascular substitution with autogenic ECs and low antigenicity.
ECs from mongrels were obtained and multiplied in vitro, then seeded to the allogeneic vein luminal surface, which was preserved by freeze-drying radiation. The cultivated cells' secretory function was confirmed by von Willebrand factor detection. The allogeneic vascular was then transplanted into animals' necks in situ. The physical properties, EC state, and vascular structure of the allogeneic vascular grafts were studied.
The secretory function of ECs did not vary in vitro. The expression level of MHC-II antigen in freeze-dried radiation-treated vasculature was lower than normal fresh vasculature (P<0.05). ECs covered the vascular inner surface and adhered tightly after implantation. As assessed by scanning electron micrograph, most ECs adhered tightly, and the cell polarity changed in accordance with the direction of the force. Allograft blood vessels with autogenic ECs implanted showed significant decreases in both thrombosis and intimal hyperplasia.
Allograft blood vessels seeded with autogenic ECs improved the patency of small-diameter grafts in a canine model. Our study showed a significant decrease in both thrombosis and intimal hyperplasia.
由于血栓形成和内膜增生，血管移植物（直径<3.0-4.0mm）的长期通畅率非常低。一种可能的机制是内皮细胞（ECs）衬里的丧失。以前尝试重新接种 ECs 的结果不佳，原因是接种细胞丢失、严重的抗原性和低顺应性。本研究的目的是生成具有自体 ECs 和低抗原性的同种异体血管替代物。
从杂种犬中获得 ECs 并在体外扩增，然后接种到同种异体静脉管腔表面，该表面通过冷冻干燥辐射保存。通过检测血管性血友病因子来确认培养细胞的分泌功能。然后将同种异体血管原位移植到动物颈部。研究了同种异体血管移植物的物理性质、EC 状态和血管结构。
ECs 的分泌功能在体外没有变化。冷冻干燥辐射处理的血管中 MHC-II 抗原的表达水平低于正常新鲜血管（P<0.05）。ECs 覆盖血管内表面，植入后紧密附着。扫描电子显微镜评估显示，大多数 ECs 紧密附着，细胞极性随着力的方向发生变化。植入自体 ECs 的同种异体血管显示血栓形成和内膜增生均显著减少。
自体 ECs 接种的同种异体血管改善了犬模型中小直径移植物的通畅率。我们的研究显示血栓形成和内膜增生均显著减少。
