Federal University of Uberlandia - Institute of Biomedical Sciences - Laboratory of Parasitology, Av. Pará, 1720 - Umuarama Campus, Minas Gerais, Brazil.
Vet Parasitol. 2010 Mar 25;168(3-4):185-9. doi: 10.1016/j.vetpar.2009.11.017. Epub 2009 Dec 16.
This study aimed at measuring intestinal villi and assessing the intestinal absorptive area in broilers infected with Eimeria acervulina under different treatments to control coccidiosis. The experiment was divided into two stages, carried out in successive housings, raised in the same environment (or aviary). In the first stage, on 25 May 2008, fifty 12-day-old birds were orally inoculated with 3 x 10(3) oocysts of E. acervulina. In the second stage, on July 2008, other 50 birds were allocated on litter contaminated by the feces of birds on the first housing (natural infection by oocysts present in the reused litter). The experiment was arranged in a complete randomized design with five treatments and three replicates of 10 chicks per treatment. Broiler chicks were housed at 1 day of age and autopsies were performed at 21 days of age. Three 2-cm-long segments of the duodenum were excised from each bird and fixed in 10% buffered formalin. A total of 30 slides were prepared for each treatment, totaling 150 evaluated histological sections using H&E staining. Villus morphology was carried out by the HL Image 97 software. The intestinal absorptive area was calculated and macroscopic lesions were classified according to standard lesion scores. Results showed that intestinal villus measurements and absorptive area are directly affected by E. acervulina and that there is direct and positive correlation between the macro and microscopic findings observed in intestinal coccidiosis. E. acervulina causes shortening of villi and reduction in the intestinal absorptive area, affecting broiler growth. The prevention method of litter fermentation during the interval between housings and oral administration of Diclazuril can reduce the severity of intestinal lesions by E. acervulina in broilers impairing oocyst virulence or viability.
本研究旨在测量感染堆型艾美耳球虫（Eimeria acervulina）的肉鸡的肠绒毛，并评估其肠道吸收面积，以研究不同球虫病防治措施的效果。实验分为两个阶段，在连续的鸡舍中进行，在相同的环境（或鸡舍）中饲养。在第一阶段，于 2008 年 5 月 25 日，将 50 只 12 日龄的鸡通过口服接种 3 x 10(3)个堆型艾美耳球虫卵囊。在第二阶段，于 2008 年 7 月，将另外 50 只鸡分配到第一个鸡舍中鸡粪污染的垫料上（通过重复使用垫料中存在的卵囊进行自然感染）。实验采用完全随机设计，分为 5 个处理组，每个处理组有 3 个重复，每个重复有 10 只鸡。肉鸡在 1 日龄时入舍，21 日龄时进行剖检。从每只鸡中取出 3 段长 2 厘米的十二指肠，用 10%缓冲福尔马林固定。每个处理组制备 30 张切片，共制备 150 张用于 H&E 染色的评估组织学切片。使用 HL Image 97 软件进行绒毛形态学分析。计算肠道吸收面积，并根据标准病变评分对宏观病变进行分类。结果表明，堆型艾美耳球虫直接影响肠绒毛的测量和吸收面积，并且在肠道球虫病中观察到的宏观和微观发现之间存在直接的正相关关系。堆型艾美耳球虫会导致绒毛缩短和肠道吸收面积减少，从而影响肉鸡的生长。在鸡舍之间的间隔期间进行垫料发酵和口服地克珠利的预防措施可以降低堆型艾美耳球虫对肉鸡的肠道病变的严重程度，从而降低卵囊的毒力或活力。
