Department of Animal Sciences, University of Illinois, Urbana, USA 61801.
Department of Animal Sciences, University of Illinois, Urbana, USA 61801.
Poult Sci. 2020 Dec;99(12):6559-6568. doi: 10.1016/j.psj.2020.09.046. Epub 2020 Sep 29.
A 28-day experiment was conducted in broilers to study the effects of feeding methylsulfonylmethane (MSM) and IL-10-neutralizing antibody from dried egg product (DEP) on the growth performance, immune responsivity, oxidative stress parameters, and gut health outcomes during a mild infection with mixed species of Eimeria. A total of 500 male Ross 308 chicks were allocated to five treatments: sham-inoculated (uninfected) chickens fed control diet (UCON), Eimeria-infected chickens fed control diet (ICON), and Eimeria-infected chickens fed control diet supplemented with 287 U/tonne of DEP (I-DEP), 0.4% MSM, or their combination (I-DEP-MSM), with 10 replicate cages of 10 birds per treatment. All infected groups received 1 mL of an oral inoculum containing Eimeria acervulina (10,000 oocysts), Eimeria maxima (5,000 oocysts), and Eimeria tenella (5,000 oocysts) on study days 7 and 14. Data were analyzed as a two-way ANOVA for all treatments including Eimeria-infected groups, in addition to a single degree of freedom contrast to compare uninfected and infected groups receiving the control diet. Mild Eimeria infection did not influence the growth performance in ICON compared with UCON at any time points. Overall (day 0-28) growth performance parameters were not influenced by either infection or dietary supplementation of MSM or DEP. However, birds in I-DEP-MSM showed improved ADG during study day 7 to 14 (i.e., 7 d after primary inoculation) indicating a beneficial effect immediately after Eimeria infection. Although MSM supplementation reduced thiobarbituric acid reactive substances (day 21 and 28), both MSM and DEP improved the total antioxidant capacity (day 21) in the plasma of infected birds. Histopathological outcomes were not influenced by treatments, and fecal oocyst output was higher in MSM- and DEP-supplemented groups than with ICON, indicating no beneficial effects. Similarly, expression of cecal inflammatory cytokines (IL-10, IL-1β, and interferon-γ) was not affected by MSM, DEP, or their combination. Overall, the current results suggest that both MSM and DEP supplementation may benefit birds during a mild Eimeria infection as indicated by improvements in ADG and oxidative stress outcomes.
一项为期 28 天的肉鸡试验研究了在轻度混合种艾美耳球虫感染期间，饲粮中添加甲磺酰甲烷（MSM）和抗白细胞介素-10 中和抗体（DEP）对生长性能、免疫反应、氧化应激参数和肠道健康的影响。将 500 只雄性罗斯 308 小鸡分为 5 个处理组：假感染（未感染）鸡饲粮对照组（UCON）、感染鸡饲粮对照组（ICON）、感染鸡饲粮对照组添加 287 U/吨 DEP（I-DEP）、0.4% MSM 或二者组合（I-DEP-MSM），每个处理组设 10 个重复笼，每个重复笼 10 只鸡。所有感染组在试验第 7 天和第 14 天经口接种 1ml 含柔嫩艾美耳球虫（10000 个卵囊）、巨型艾美耳球虫（5000 个卵囊）和堆型艾美耳球虫（5000 个卵囊）的混合孢子化卵囊液。对所有处理组（包括感染组）进行了双向方差分析，此外，还进行了一项单自由度对比，以比较未感染组和感染组饲粮对照组。在任何时间点，轻度艾美耳球虫感染都未影响 ICON 鸡的生长性能，与 UCON 鸡相比。总的来说（0-28 日龄），感染或饲粮添加 MSM 或 DEP 都不影响生长性能参数。然而，I-DEP-MSM 组鸡在 7-14 日龄（即初次接种后 7 天）ADG 提高，表明在感染后立即产生有益作用。虽然 MSM 补充剂降低了第 21 天和第 28 天的硫代巴比妥酸反应物（TBARS），但 MSM 和 DEP 均提高了感染鸡血浆中的总抗氧化能力（第 21 天）。组织病理学结果不受处理影响，MSM 和 DEP 组的粪便卵囊排出量高于 ICON 组，表明没有有益效果。同样，MSM、DEP 或二者组合对盲肠炎性细胞因子（白细胞介素-10、白细胞介素-1β和干扰素-γ）的表达没有影响。总的来说，目前的结果表明，MSM 和 DEP 补充剂可能对感染轻度艾美耳球虫的鸡有益，这表现在 ADG 和氧化应激结果的改善上。
