[乳胶免疫比浊法测定血清纤维蛋白降解产物及其免疫印迹分析]

[Serum FDP assay with latex photometric immunoassay and its analysis by immunoblotting].

作者信息

Okumura N, Kagiya H, Saito H, Katsuyama T, Kanai M

机构信息

Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.

出版信息

Rinsho Byori. 1991 Jan;39(1):76-82.

PMID:2008047

Abstract

Sixty patients' sera with FDP-E value ranged from 200 to 2,500 ng/ml by LPIA system were assayed for FDP-Total (FDP-T) by the same system and FDP-DD by ELISA. Correlations of values between FDP-E, FDP-T and FDP-DD were excellent and ratio of FDP-T/FDP-E (T/E) and FDP-DD/FDP-E (DD/E) from same 60 sera were 32.4 + 7.4 and 1.55 + 0.50, respectively. Ratio of T/E from sera of FDP-E value demonstrating more than 500 ng/ml were classified into three groups, namely, 25.0-35.0, less than 18.0, and more than 50.0. Ten sera in each group were analyzed for FDP fragments of D, DD, Y, DY or X (DY/X) and high molecular weight (HMW) with SDS-PAGE and immunoblot method. It was found that relative concentration and proportion of D and DD.E fragments showed its characteristic pattern in each group.

摘要

通过乳胶凝集免疫测定法（LPIA系统）对60例FDP-E值在200至2500 ng/ml之间的患者血清进行检测，用同一系统测定FDP总量（FDP-T），并用酶联免疫吸附测定法（ELISA）测定FDP-DD。FDP-E、FDP-T和FDP-DD之间的值相关性良好，来自同一60份血清的FDP-T/FDP-E（T/E）和FDP-DD/FDP-E（DD/E）比值分别为32.4±7.4和1.55±0.50。FDP-E值大于500 ng/ml的血清T/E比值分为三组，即25.0 - 35.0、小于18.0和大于50.0。采用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法对每组中的10份血清进行D、DD、Y、DY或X（DY/X）以及高分子量（HMW）的FDP片段分析。结果发现，每组中D和DD.E片段的相对浓度和比例呈现出其特征性模式。