经表生毛孢子菌（Kano）DA5501 细胞在正十二烷上生长，将 2,6-二甲基吡啶生物转化为 6-甲基烟酸。
Bioconversion of 2,6-dimethylpyridine to 6-methylpicolinic acid by Exophiala dermatitidis (Kano) de Hoog DA5501 cells grown on n-dodecane.
机构信息
Department of Biomolecular Science, Faculty of Engineering, Gifu University, Yanagido 1-1, Gifu, Japan.
出版信息
Appl Microbiol Biotechnol. 2010 Apr;86(4):1165-70. doi: 10.1007/s00253-009-2419-4. Epub 2010 Jan 16.
Alkane-assimilating microorganisms were isolated from enrichment cultures using n-octane, n-dodecane, n-hexadecane, or pristane (2,6,10,14-tetramethylpentadecane) as a sole carbon source to find microbial catalysts oxidizing methyl groups of 2,6-dimethylpyridine. The cells of Exophiala dermatitidis (Kano) de Hoog DA5501, an n-dodecane-assimilating fungus, oxidized a single methyl group of 2,6-dimethylpyridine to produce 6-methylpicolinic acid (6-methylpyridine-2-carboxylic acid) without the formation of dipicolinic acid (pyridine-2,6-dicarboxylic acid); 67 mM 6-methylpicolinic acid (9.2 g/l) accumulated with a molar conversion yield of 89% by 54-h incubation. The fungus cells also oxidized the methyl group of 2,6-dimethylpyrazine and 2,4,6-trimethylpyridine regioselectively.
从利用正辛烷、正十二烷、正十六烷或姥鲛烷（2,6,10,14-四甲基十五烷）作为唯一碳源的富集培养物中分离出了烷烃同化微生物，以寻找微生物催化剂来氧化 2,6-二甲基吡啶的甲基基团。一种能够同化正十二烷的真菌——外瓶霉（Kano）de Hoog DA5501 的细胞，将 2,6-二甲基吡啶的单个甲基基团氧化生成 6-甲基烟酸（6-甲基吡啶-2-羧酸），而不形成二烟酸（吡啶-2,6-二羧酸）；在 54 小时的孵育过程中，积累了 67mM 的 6-甲基烟酸（9.2g/L），摩尔转化率为 89%。真菌细胞还能选择性地氧化 2,6-二甲基吡嗪和 2,4,6-三甲基吡啶的甲基基团。
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