Department of Microbiology, N.V. Patel college of Pure and Applied Sciences, Sardar Patel University, Vallabh-Vidyanagar, Gujarat, India.
J Basic Microbiol. 2010 Feb;50(1):43-51. doi: 10.1002/jobm.200900203.
Laccase production by solid state fermentation (SSF) using an indigenously isolated litter dwelling fungus Fusarium incarnatum LD-3 was optimized. Fourteen medium components were screened by the initial screening method of Plackett-Burman. Each of the components was screened on the basis of 'p' (probability value) which was above 95% confidence level. Ortho-dianisidine, thiamine HCl and CuSO(4) . 5 H(2)O were identified as significant components for laccase production. The Central Composite Design response surface methodology was then applied to further optimize the laccase production. The optimal concentration of these three medium components for higher laccase production were (g/l): CuSO(4) . 5 H(2)O, 0.01; thiamine HCl, 0.0136 and ortho-dianisidine, 0.388 mM served as an inducer. Wheat straw, 5.0 g was used as a solid substrate. Using this statistical optimization method the laccase production was found to increase from 40 U/g to 650 U/g of wheat straw, which was sixteen times higher than non optimized medium. This is the first report on statistical optimization of laccase production from Fusarium incarnatum LD-3.
采用固态发酵（SSF）技术，利用本土分离的真菌密环菌 LD-3 生产漆酶。采用 Plackett-Burman 初始筛选法筛选出 14 种培养基成分。基于置信水平为 95%的“p”值（概率值）对每种成分进行筛选。邻二茴香胺、盐酸硫胺素和 CuSO₄·5H₂O 被鉴定为漆酶生产的重要成分。然后应用中心复合设计响应面法进一步优化漆酶生产。这三种培养基成分的最佳浓度（g/L）为：CuSO₄·5H₂O 为 0.01；盐酸硫胺素为 0.0136；邻二茴香胺为 0.388 mM，作为诱导剂。麦秸 5.0 g 用作固体基质。使用这种统计优化方法，漆酶产量从 40 U/g 提高到 650 U/g 麦秸，比非优化培养基提高了 16 倍。这是密环菌 LD-3 生产漆酶的统计优化的首次报道。
