Trivedi Janki, Chhaya Urvish
Department of Microbiology, N.V. Patel College of Pure and Applied Sciences, Vallabh Vidyanagar, Anand, Gujarat India.
3 Biotech. 2021 Jun;11(6):297. doi: 10.1007/s13205-021-02842-4. Epub 2021 May 25.
In recent times, there is increased public interest and indeed strong movement against the use of Bisphenol A (4,4'-(propane-2,2,-diphenol)) due to its endocrine disrupting properties. In the present study, biotransformation of Bisphenol A (BPA) was accomplished using laccase (E.C. 1.10.3.2) enzyme. The enzyme was entrapped in reverse micelles comprising of bis(2-ethylhexyl) sulfosuccinate sodium salt (AOT) and 2,2,4-trimethylpentane (isooctane) for non-aqueous catalysis considering hydrophobicity of BPA. Screening of various parameters that may affect micellar system was carried out using Plackett-Burman experimental design and central composite design (Design Expert 11). According to Design Expert actual concentration of different variables was 0.55, 150 (Wo 30), 0.0035 mM and 175 µg/ml for Mgions, Hydration ratio (Wo), 2,6-dimethoxyphenol (2,6 DMP, substrate) and laccase, respectively, at 40 °C and pH 4.5. Under these conditions laccase activity in reverse micelles was increased two folds as compared to unoptimized micellar system. It was evident that the reverse micelles diameter was linearly proportionated to the amount of laccase enzyme incorporated. BPA bioremediation mediated by laccase in non-aqueous environment was found to be 84% in 8 h of treatment. Biotransformation of BPA was monitored using GC-MS. BPA degraded products, such as BPA-O-catechol and 4,4 (Ethane 2-oxy 2-ol) diphenol were identified indicating transformation by oxidation.
The online version contains supplementary material available at 10.1007/s13205-021-02842-4.
近年来，由于双酚A（4,4'-（丙烷-2,2-二酚））具有内分泌干扰特性，公众对其使用的关注度日益提高，且确实有强烈的抵制行动。在本研究中，使用漆酶（E.C. 1.10.3.2）实现了双酚A（BPA）的生物转化。考虑到BPA的疏水性，将该酶包埋在由双（2-乙基己基）磺基琥珀酸钠盐（AOT）和2,2,4-三甲基戊烷（异辛烷）组成的反胶束中进行非水催化。使用Plackett-Burman实验设计和中心复合设计（Design Expert 11）对可能影响胶束系统的各种参数进行筛选。根据Design Expert，在40°C和pH 4.5条件下，Mg离子、水合比（Wo）、2,6-二甲氧基苯酚（2,6 DMP，底物）和漆酶的不同变量实际浓度分别为0.55、150（Wo 30）、0.0035 mM和175 μg/ml。在这些条件下，与未优化的胶束系统相比，反胶束中的漆酶活性提高了两倍。很明显，反胶束直径与包埋的漆酶量呈线性比例关系。发现在非水环境中由漆酶介导的BPA生物修复在处理8小时后为84%。使用GC-MS监测BPA的生物转化。鉴定出BPA降解产物，如BPA-O-儿茶酚和4,4（乙烷2-氧基2-醇）二酚，表明通过氧化发生了转化。
在线版本包含可在10.1007/s13205-021-02842-4获取的补充材料。
