Department of Neurosurgery, Institute of Neuroscience of Guangdong Province, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China.
Technol Cancer Res Treat. 2010 Feb;9(1):87-94. doi: 10.1177/153303461000900110.
Argon-helium cryosurgery has shown encouraging therapeutic effects on some solid tumors, but its application in the treatment of gliomas remains poorly documented. To explore the cell apoptosis at the glioma foci and the cellullar immunity changes following argon-helium cryosurgery, we established Wistar rat models bearing subcutaneous C6 glioma and divided the rats into the normal control (20 rats), sham-operated (32 rats), surgical resection (20 rats), and cryosurgery (32 rats) groups with corresponding treatments. The postoperative changes in the findings by magnetic resonance imaging (MRI) and tumor cell morphology were observed, and the cell apoptosis at the tumor foci was assessed with TUNEL assay. Flow cytometry was performed for analysis of the T lymphocyte subset changes following the cryosurgery. The results showed that cryosurgery induced not only destruction of the tumor cells but also cell apoptosis around the cryablation foci. Compared with surgical resction that caused significant reduction in CD3+ and CD4+ cell percentages, cryosurgery resulted in significantly increased percentages of CD3+ and CD4+ cells (P<0.05) with a relative increase of the CD4+/CD8+ cell ratio 14 days after the operation. These results demonstrate that in addition to tumor cell destruction, cryosurgy also results in enhanced cellular immunity and antitumor immune response of the C6 glioma-bearing rats, suggesting the great potential of argon-helium cryosurgery in clinical management of gliomas.
氩氦刀冷冻治疗在某些实体肿瘤的治疗中已显示出令人鼓舞的疗效，但在脑胶质瘤治疗中的应用仍缺乏充分的文献记载。为了探讨氩氦刀冷冻治疗后脑胶质瘤病灶细胞凋亡及细胞免疫变化，我们建立了 Wistar 大鼠皮下 C6 脑胶质瘤模型，将大鼠分为正常对照组（20 只）、假手术组（32 只）、手术切除组（20 只）和冷冻治疗组（32 只），并给予相应处理。观察术后磁共振成像（MRI）表现及肿瘤细胞形态学的变化，采用 TUNEL 法检测肿瘤细胞凋亡。采用流式细胞术检测冷冻治疗后 T 淋巴细胞亚群的变化。结果表明，氩氦刀冷冻治疗不仅可导致肿瘤细胞破坏，而且可诱导肿瘤冷冻消融灶周围细胞凋亡。与手术切除组相比，冷冻治疗组可明显提高 CD3+和 CD4+细胞的比例（P<0.05），14 天时 CD4+/CD8+比值明显升高，且 CD3+和 CD4+细胞比例降低更为明显。这些结果表明，氩氦刀冷冻治疗除了能破坏肿瘤细胞外，还能增强 C6 脑胶质瘤荷瘤大鼠的细胞免疫和抗肿瘤免疫反应，提示氩氦刀冷冻治疗在脑胶质瘤临床治疗中有很大的应用潜力。
