Centro de Investigación y de Estudios Avanzados del IPN, Av. IPN, Col. San Pedro Zacatenco, México, D.F., México.
Arch Med Res. 2009 Oct;40(7):545-50. doi: 10.1016/j.arcmed.2009.10.002.
There is an increasing interest about the effects of electromagnetic fields on health and clinical applications. Electromagnetic fields have been shown to promote differentiation and regeneration of many tissues. The purpose of the present study was to evaluate if a magnetic field (MF) varying in time is able to induce neurite outgrowth in cultured chromaffin cells. For this reason, a stimulation system was developed in order to generate a magnetic field, using permanent magnets as a supply.
In this investigation we used a pair of permanent ferrite magnets. These were mounted in a mechanical system in which both magnets rotate around a culture Petri dish. The stimulation device was designed at Centro de Investigación y de Estudios Avanzados, Avanzados del IPN, Mexico City. Primary cultures of chromaffin cells were stimulated with a magnetic field of 6.4 mT and 4, 7, 10 or 12Hz (2h daily, during a 7-day period). After treatment, percentage of neurite outgrowth was calculated.
Our results show that the magnetic fields produced by rotating permanent magnets induced neurite outgrowth on chromaffin cells at 7 and 10Hz.
The present study provides evidence that MFs varying in time (7 and 10Hz) induce neurite outgrowth in chromaffin cells. These studies will contribute to elucidate the effect of noninvasive MF stimulus in order to apply it in future regeneration therapies. Also, the device designed could be used for different kind of cells and may work as a model for future clinical devices.
电磁场对健康和临床应用的影响越来越受到关注。电磁场已被证明能促进许多组织的分化和再生。本研究的目的是评估时变电磁场是否能诱导培养的嗜铬细胞产生突起。为此，我们开发了一种刺激系统，使用永磁体作为电源产生磁场。
在这项研究中，我们使用了一对永磁体。这些永磁体被安装在一个机械系统中，在这个系统中，两个永磁体绕着培养皿旋转。刺激装置是由墨西哥城先进研究与高级研究所的 Centro de Investigación y de Estudios Avanzados 设计的。用 6.4mT 的磁场和 4、7、10 或 12Hz（每天 2 小时，持续 7 天）刺激原代培养的嗜铬细胞。处理后，计算突起生长的百分比。
我们的结果表明，旋转永磁体产生的磁场能诱导 7Hz 和 10Hz 时嗜铬细胞的突起生长。
本研究提供了证据表明，时变磁场（7Hz 和 10Hz）能诱导嗜铬细胞的突起生长。这些研究将有助于阐明非侵入性磁场刺激的效果，以便将其应用于未来的再生治疗。此外，设计的装置可用于不同类型的细胞，并可作为未来临床设备的模型。
