Australian Centre for Research on Separation Science, School of Applied Sciences, RMIT University, G.P.O. Box 2476, Melbourne 3001, Australia.
J Chromatogr A. 2010 Feb 26;1217(9):1522-9. doi: 10.1016/j.chroma.2009.12.078. Epub 2010 Jan 4.
In this study, a new system for analysis using a dual comprehensive two-dimensional gas chromatography/targeted multidimensional gas chromatography (switchable GC x GC/targeted MDGC) analysis was developed. The configuration of this system not only permits the independent operation of GC, GC x GC and targeted MDGC analyses in separate analyses, but also allows the mode to be switched from GC x GC to targeted MDGC any number of times through a single analysis. By incorporating a Deans switch microfluidics transfer module prior to a cryotrapping device, the flow stream from the first dimension column can be directed to either one of two second dimension columns in a classical heart-cutting operation. Both second columns pass through the cryotrap to allow solute bands to be focused and then rapidly remobilized to the respective second columns. A short second column enables GC x GC operation, whilst a longer column is used for targeted MDGC. Validation of the system was performed using a standard mixture of compounds relevant to essential oil analysis, and then using compounds present at different abundances in lavender essential oil. Reproducibility of retention times and peak area responses demonstrated that there was negligible variation in the system over the course of multiple heart-cuts, and proved the reliable operation of the system. An application of the system to lavender oil, as a more complex sample, was carried out to affirm system feasibility, and demonstrate the ability of the system to target multiple components in the oil. The system was proposed to be useful for study of aroma-impact compounds where GC x GC can be incorporated with MDGC to permit precise identification of aroma-active compounds, where heart-cut multidimensional GC-olfactometry detection (MDGC-O) is a more appropriate technology for odour assessment.
在本研究中，开发了一种新的分析系统，该系统使用双全二维气相色谱/靶向多维气相色谱（可切换 GC x GC/靶向 MDGC）分析。该系统的配置不仅允许 GC、GC x GC 和靶向 MDGC 分析在单独的分析中独立运行，而且还允许通过单次分析多次从 GC x GC 切换到靶向 MDGC 模式。通过在冷阱装置前采用Dean 开关微流控传输模块，可将第一维柱的流动相引导至两个二维柱中的任一个进行经典的中心切割操作。两根第二根柱都通过冷阱，允许溶质带聚焦，然后迅速重新移动到各自的第二根柱。短的第二根柱可用于 GC x GC 操作，而较长的柱则用于靶向 MDGC。使用与精油分析相关的标准化合物混合物对系统进行验证，然后使用薰衣草精油中存在的不同丰度的化合物进行验证。保留时间和峰面积响应的重现性表明，在多次中心切割过程中，系统几乎没有变化，证明了系统的可靠运行。该系统在薰衣草油中的应用是一个更复杂的样本，以确认系统的可行性，并证明该系统靶向油中多个成分的能力。该系统被提议用于研究香气影响化合物，其中 GC x GC 可与 MDGC 结合使用，以允许对香气活性化合物进行精确鉴定，而中心切割多维气相色谱-嗅觉检测（MDGC-O）是更适合气味评估的技术。
