Department of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland.
Bioelectrochemistry. 2010 Aug;79(1):90-4. doi: 10.1016/j.bioelechem.2009.12.006. Epub 2009 Dec 16.
The effectiveness of the photodynamic therapy (PDT), a low-invasive and targeted therapy of cancer, could be intensified by increasing the intracellular transport of a photosensitizer. The electroporation is used to generate non-specific transient nanopores that facilitate local drug delivery into cells. Photodynamic therapy assisted by electroporation was tested in vitro on the human lung carcinoma cell line A549 by determining the mitochondrial cell function using the MTT assay. The photodynamic activity of the electro-photodynamic treatment (EPDT) with the hematoporphyrin derivative was evaluated in relation to the photodynamic method alone. The experiments show significantly increased efficiency of EPDT, which allows reducing drug doses and exposure time of the cells to the drug in standard PDT. The results have been confronted with the model based on van't Hoff equation. This showed that the growth fractions of cells after EPDT depend on the electric field according to the same relation as in case of electroporation alone.
光动力疗法（PDT）是一种低侵入性和靶向性的癌症治疗方法，通过增加光敏剂的细胞内转运，可以增强其疗效。电穿孔用于产生非特异性瞬时纳米孔，从而促进局部药物递送到细胞内。通过 MTT 测定法测定线粒体细胞功能，在体外用人肺癌细胞系 A549 上测试了电穿孔辅助的光动力疗法。评估了血卟啉衍生物的电-光动力疗法（EPDT）与单纯光动力疗法的光动力活性。实验表明，EPDT 的效率显著提高，这使得可以减少药物剂量并缩短细胞暴露于标准 PDT 药物的时间。将结果与基于范特霍夫方程的模型进行了比较。结果表明，EPDT 后细胞的生长分数取决于电场，这与单独电穿孔的情况相同。
