Perry R R, Matthews W, Mitchell J B, Russo A, Evans S, Pass H I
Thoracic Oncology Section, Surgery Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892.
Cancer Res. 1990 Jul 15;50(14):4272-6.
The relative sensitivities of different cancer histologies in a single site to photodynamic therapy (PDT) are unknown and methods to predict PDT sensitivity have not been described. The in vitro response to PDT of six established human lung cancer cell lines and one normal lung fibroblast cell line was studied using the clonogenic assay. Dose-response curves were determined for cells incubated in 25 micrograms/ml of Photofrin II for 2 h, followed by exposure to 630-nm light to total energies of 0-3150 J/m2. None of the cell lines were sensitive to sensitizer alone or light alone. Differences in inherent PDT sensitivities as evaluated by survival curve parameters n, Do, and light dose to yield 1% survival were observed among the cell lines. No clear correlation was found when inherent PDT sensitivity was compared with sensitizer uptake; however, a general association was noted between PDT sensitivity and the plating efficiency of the cell line. These data illustrate that differences in inherent PDT exist for in vitro systems. Such differences may explain some of the failures seen in clinical PDT.
单一部位不同癌症组织学对光动力疗法（PDT）的相对敏感性尚不清楚，且尚未描述预测PDT敏感性的方法。使用克隆形成试验研究了六种已建立的人肺癌细胞系和一种正常肺成纤维细胞系对PDT的体外反应。将细胞在25微克/毫升的光敏素II中孵育2小时，然后暴露于630纳米的光下，总能量为0 - 3150焦/平方米，测定剂量反应曲线。所有细胞系对单独的敏化剂或单独的光均不敏感。在细胞系之间观察到通过存活曲线参数n、Do和产生1%存活的光剂量评估的固有PDT敏感性差异。当将固有PDT敏感性与敏化剂摄取进行比较时，未发现明显相关性；然而，注意到PDT敏感性与细胞系的接种效率之间存在一般关联。这些数据表明体外系统存在固有PDT差异。这种差异可能解释了临床PDT中出现的一些失败情况。
