Southern Plains Agricultural Research Center, Agricultural Research Service, U.S. Department of Agriculture, College Station, Texas, USA.
Innate Immun. 2011 Apr;17(2):137-44. doi: 10.1177/1753425909356937. Epub 2010 Jan 18.
Toll-like receptors (TLRs) recognize microbial components and initiate the innate immune responses that control microbial infections. The interaction between ligands of TLR3 and TLR9, poly I:C (an analog of viral double-stranded RNA) and CpG-ODN (a CpG-motif containing oligodeoxydinucleotide) on the inflammatory immune responses, including the production of nitric oxide (NO) and the expression of inducible NO synthase (iNOS), pro-inflammatory cytokines interleukin (IL)-1β and IL-6, and chemokines IL-8 and macrophage inflammatory protein (MIP)-1β, were investigated in chicken monocytes. The NO production was significantly higher when stimulated with a combination of CpG-ODN and poly I:C than with either CpG-ODN or poly I:C alone. Similarly, a significant synergistic effect by CpG-ODN and poly I:C was observed in the up-regulation of iNOS and IL-8 mRNA after 2 h and persisted up to 24 h. Although the combinatory treatment of CpG-ODN and poly I:C enhanced the expression of IL-1β, IL-6, and MIP-1β(3 after 2 h stimulation, the synergism in the up-regulation of IL-1β and IL-6 mRNA was observed after 8-h and 24-h stimulation, respectively, whereas there was no synergistic effect on MIP-1β. Our results demonstrate that CpG-ODN synergizes with poly I:C to induce pro-inflammatory immune response in chicken monocytes.
Toll 样受体 (TLRs) 识别微生物成分并启动固有免疫反应，以控制微生物感染。TLR3 和 TLR9 的配体（聚肌苷酸和 CpG-ODN）与鸡单核细胞中的炎症免疫反应相互作用，包括一氧化氮 (NO) 的产生和诱导型一氧化氮合酶 (iNOS)、促炎细胞因子白细胞介素 (IL)-1β 和 IL-6 以及趋化因子 IL-8 和巨噬细胞炎症蛋白 (MIP)-1β 的表达。当用 CpG-ODN 和聚肌苷酸的组合刺激时，NO 的产生明显高于单独用 CpG-ODN 或聚肌苷酸刺激时。同样，在 2 小时后 iNOS 和 IL-8 mRNA 的上调中观察到 CpG-ODN 和聚肌苷酸的协同作用，并持续到 24 小时。虽然 CpG-ODN 和聚肌苷酸的联合治疗增强了 IL-1β、IL-6 和 MIP-1β 的表达（在 2 小时刺激后），但在 8 小时和 24 小时刺激后观察到 IL-1β 和 IL-6 mRNA 的上调协同作用，而 MIP-1β 则没有协同作用。我们的结果表明，CpG-ODN 与聚肌苷酸协同诱导鸡单核细胞中的促炎免疫反应。
