Department of Histology, Medical University of Gdansk, Gdansk, Poland.
J Physiol Pharmacol. 2009 Oct;60 Suppl 4:57-62.
FHIT gene encodes human diadenosine triphosphate hydrolase involved in the regulation of cell cycle and nucleotide metabolism and is a candidate tumor suppressor gene.
To investigate expression of FHIT gene at the mRNA and protein levels in sporadic inflammatory bowel disease (IBD).
FHIT mRNA was quantified by the validated real-time PCR (QPCR) and FHIT protein was detected by immunohistochemistry (IHC) in mucosal biopsies of 139 ulcerative colitis (UC), 19 Crohn's disease (CD) and 37 control patients.
Significant FHIT gene overexpression was found in 78% of active UC but not in CD. IHC showed comparable results to QPCR.
The local up-regulation of FHIT gene and protein expression in active UC may represent an adequate response against inflammatory challenge of epithelial cell homeostasis and protect against DNA damage and cell cycle disturbances.
脆性组氨酸三联体（FHIT）基因编码人类二腺苷三磷酸水解酶，参与细胞周期和核苷酸代谢的调节，是候选肿瘤抑制基因。
研究散发性炎症性肠病（IBD）中 FHIT 基因在 mRNA 和蛋白水平的表达。
采用已验证的实时 PCR（QPCR）定量检测 139 例溃疡性结肠炎（UC）、19 例克罗恩病（CD）和 37 例对照患者黏膜活检组织中的 FHIT mRNA，采用免疫组织化学（IHC）检测 FHIT 蛋白。
在活动期 UC 中发现 FHIT 基因过度表达，阳性率为 78%，而在 CD 中则没有。IHC 结果与 QPCR 结果一致。
在活动期 UC 中，FHIT 基因和蛋白表达的局部上调可能代表了对上皮细胞稳态炎症挑战的适当反应，并防止了 DNA 损伤和细胞周期紊乱。
