Pitzel L, Nazar S, Stinshoff D, Semler P, Rösler-Englhardt A
MMW Munch Med Wochenschr. 1977 Oct 7;119(40):1283-8.
Different methods for determination and classification systems of serum lipoproteins were used and compared in 300 patients with hyperlipidemia. The procedures and methods were examined to find out which would provide a useful classification for diagnosis and therapy. Lipid electrophoresis on agarose gel gave no reliably reproducible typing. With acetate and polyacetate foils, Type llb was not recognizable in the majority of cases. Neither the determination of beta-cholesterol after polyanion precipitation nor quantitative lipid electrophoresis calculating the values as lipoprotein lipid gave type determinations which completely agreed with the ultracentrifuge, but the best coincidence with the ultracentrifuge was obtained with these two methods. The second method is chiefly suitable for supervision.
在300例高脂血症患者中，使用并比较了血清脂蛋白的不同测定方法和分类系统。对这些程序和方法进行了检查，以找出哪种方法能为诊断和治疗提供有用的分类。琼脂糖凝胶上的脂质电泳无法得到可靠的可重复分型。使用醋酸盐和聚醋酸盐箔时，大多数情况下无法识别Ⅱb型。聚阴离子沉淀后测定β-胆固醇以及定量脂质电泳（将值计算为脂蛋白脂质）都无法给出与超速离心完全一致的分型结果，但这两种方法与超速离心的一致性最好。第二种方法主要适用于监测。
