Department of Veterinary Science, The University of Melbourne, Werribee, Victoria, Australia.
Electrophoresis. 2010 Jan;31(2):287-98. doi: 10.1002/elps.200900480.
This study explored the genetic composition of Giardia in fecal samples from 284 individual lambs on pasture-based sheep farms in three regions of Victoria, Australia. An approach, combining targeted sequencing, phylogenetic analysis and PCR-coupled restriction endonuclease fingerprinting, was used to identify and genetically categorize Giardia present in 43 (15.1%) of the 284 samples and to infer their zoonotic potential. The specific identity and genetic classification were based on the phylogenetic analysis of sequence data for a portion of the triose-phosphate isomerase gene. Fourteen different sequence variants (including seven sequences that contained between one and five polymorphic sites) representing two distinct assemblages of Giardia (recognized in the current literature) were defined, of which 13 were new records. One dominant sequence type (with accession no. GQ444447, representing a genotype within assemblage A) has been detected previously in humans and is thus considered to be of zoonotic potential. (Nucleotide sequences reported in this article are available in the GenBank database under accession nos. GQ444447-GQ444451 and GQ444454-GQ444462).
本研究探索了澳大利亚维多利亚州三个地区基于牧场的绵羊养殖场 284 只个体羔羊粪便样本中的贾第虫的基因组成。采用了一种结合靶向测序、系统发育分析和 PCR 耦联限制性内切酶指纹分析的方法，鉴定并对 284 个样本中的 43 个（15.1%）样本中存在的贾第虫进行基因分类，并推断其人畜共患潜力。特定的身份和遗传分类是基于三磷酸甘油醛异构酶基因部分序列数据的系统发育分析。定义了 14 种不同的序列变体（包括包含 1 到 5 个多态性位点的 7 个序列），代表贾第虫的两个不同的集合（在当前文献中被认可），其中 13 个是新记录。一种主要的序列类型（ accession no. GQ444447，代表集合 A 中的一个基因型）以前在人类中检测到，因此被认为具有人畜共患潜力。（本文报道的核苷酸序列可在 GenBank 数据库中获得，登录号为 GQ444447-GQ444451 和 GQ444454-GQ444462）。
