蓝氏贾第鞭毛虫磷酸丙糖异构酶（tim）基因的种内差异
The intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia.
作者信息
Lu Siqi, Li Jihong, Zhang Yaping, Wen Jianfan, Wang Fengyun
机构信息
Department of Cell Biology, the Capital University of Medical Sciences, Beijing 100054, China.
出版信息
Chin Med J (Engl). 2002 May;115(5):763-6.
OBJECTIVE
To investigate the intraspecific difference of the triose phosphate isomerase (tim) gene from Giardia lamblia (G. lamblia).
METHODS
Total genomic DNA of G. lamblia was extracted and partial fragments of the triose phosphate isomerase (tim) gene were amplified by polymerase chain reaction (PCR). All nucleotide sequences were analyzed by using a phylogenetic analysis, which was constructed with parsimony and Neighbor-joining (N-J) methods.
RESULTS
A total of 124 variable sites (23% of all sequences detected) was defined, most of which were found at the silent sites of codons. Two similar phylogenetic trees were constructed, subdividing 16 Giardia isolates into two groups.
CONCLUSION
The genetic diversity of G. lamblia appeared to be little affected by factors of both host and geography, while natural-selection played an important role in DNA molecular evolution level of the tim gene. The tim gene may be considered a very useful genetic marker of the population genetic structure of G. lamblia.
目的
研究蓝氏贾第鞭毛虫磷酸丙糖异构酶（tim）基因的种内差异。
方法
提取蓝氏贾第鞭毛虫的全基因组DNA，通过聚合酶链反应（PCR）扩增磷酸丙糖异构酶（tim）基因的部分片段。所有核苷酸序列均采用系统发育分析进行分析，该分析采用简约法和邻接法（N-J）构建。
结果
共定义了124个可变位点（占所有检测序列的23%），其中大部分位于密码子的沉默位点。构建了两个相似的系统发育树，将16株贾第虫分离株分为两组。
结论
蓝氏贾第鞭毛虫的遗传多样性似乎受宿主和地理因素的影响较小，而自然选择在tim基因的DNA分子进化水平中起重要作用。tim基因可被认为是蓝氏贾第鞭毛虫群体遗传结构的一个非常有用的遗传标记。
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