[山东省分离的肠道细胞病变人孤儿病毒13型的基因特征分析]
[The genetic characterization of enteric cytopathic human orphanviruses type 13 isolated in Shandong province].
作者信息
Mao Nai-ying, Wang Hai-yan, Zhu Zhen
机构信息
World Health Organization Western Pacific Region Regional Reference Measles Laboratory, State Key Laboratory for Molecular & Genetic Engineering, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
出版信息
Zhongguo Yi Miao He Mian Yi. 2009 Jun;15(3):238-41.
OBJECTIVE
To analysis the genetic characteristics of Echo 13 isolated from stool specimens of AFP patient in Shandong.
METHODS
The complete VP1 sequences of the 6 isolates were successfully amplified by RT-PCR and were compared with other Echovirus 13 isolates available from GenBank.
RESULTS
6 Shandong isolates were clearly identified as Echo 13 and were grouped into B cluster that agreed with neutralization result. The homogeneity analysis showed that the Shandong isolates share at least 80% similarity in nucleotide level and 94.4% in amino acid level. Phylogenetic tree base on complete VP1 sequence indicated there were many transmitting chains of Echovirus 13 prevailed in China.
CONCLUSION
All Echo 13 isolates were divided into three genomic clusters A, B and C. It is suggested that enterovirus surveillance should be strengthened to prevent imported of Echo 13.
目的
分析从山东 AFP 患者粪便标本中分离出的埃可 13 型病毒的基因特征。
方法
通过 RT-PCR 成功扩增出 6 株分离株的完整 VP1 序列，并与 GenBank 中其他可用的埃可病毒 13 型分离株进行比较。
结果
6 株山东分离株被明确鉴定为埃可 13 型病毒，并被归入与中和试验结果一致的 B 簇。同源性分析表明，山东分离株在核苷酸水平上至少有 80%的相似性，在氨基酸水平上有 94.4%的相似性。基于完整 VP1 序列构建的系统发育树表明，中国存在许多埃可病毒 13 型的传播链。
结论
所有埃可 13 型病毒分离株分为 A、B、C 三个基因组簇。建议加强肠道病毒监测，以防止埃可 13 型病毒的输入。
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