Sanlidağ Tamer, Akçali Sinem, Ozbakkaloğlu Beril, Ertekin Deniz, Akduman Elçin
Celal Bayar Universitesi Tip Fakültesi, Mikrobiyoloji ve Klinik Mikrobiyoloji Anabilim Dali, Manisa.
Mikrobiyol Bul. 2009 Oct;43(4):613-8.
The duration of hepatitis C virus (HCV) infection and the response to the standard therapy is strongly related to the HCV genotypes. In addition, the geographical distribution of HCV genotypes is important for the epidemiological studies in terms of distribution and possible risk groups. The aim of this retrospective study was to investigate the distribution of HCV genotypes in Manisa region (located at the Aegean part of Turkey). A total of 100 anti-HCV (microparticle EIA; Abbott Laboratories, USA) and HCV-RNA (real time RT-PCR; Applied Biosystems, USA) positive patients (53 female, 47 male; mean age: 44.4 +/- 10.4 years), who were admitted to Celal Bayar University Medical School Hospital between 2002-2005, were included to the study. Quantitative HCV-RNA levels of the patients were between 10(4)-10(8) copies/ml. Complementary DNAs obtained from HCV-RNAs isolated by Invitek RTP DNA/RNA Virus Mini Kit were used for genotyping with selected primers [primer 11 (5'-AGG TCT CTG AGA CCG TGC ACC ATG AGC AC-3') and primer 13 (5'-CTG TGA GGA ACT ACT GTC TT-3') for the first PCR; primer 12 (5'-ACT GCC TGA TAG GGT GCT TGC GAG TG-3') and primer 14 (5'-CAC GCA GAA AGC GTC TAG-3') for the second PCR]. The RT-PCR products were purified with Invisorb Spin PCRapid Kit and sequenced by BigDye Terminator v3.1 Cycle Sequencing Kit in ABI Prism 310 Genetic Analyzer. Genotype 1 was found in 92% of the patients (92%) and genotypes 2 and 4 were found in 7% of the patients, while HCV genotype could not be identified in one patient (1%). When evaluating the subtypes, genotype 1b was determined in 90 patients (90%), genotype 4a in five patients (5%), genotype 1a in two patients (2%) and genotype 2a in two patients (2%). In conclusion, 1b was found to be the most common HCV genotype in Manisa region in concordance with the previous data obtained in Turkey, followed by genotype 4a, although a rare one. The data of this study is noteworthy especially for the arrangement of treatment and follow-up of HCV infected patients.
丙型肝炎病毒（HCV）感染的持续时间以及对标准治疗的反应与HCV基因型密切相关。此外，HCV基因型的地理分布对于分布情况及可能的风险群体的流行病学研究很重要。这项回顾性研究的目的是调查马尼萨地区（位于土耳其爱琴海地区）HCV基因型的分布情况。共有100名抗-HCV（微粒酶免疫测定法；美国雅培实验室）和HCV-RNA（实时逆转录聚合酶链反应；美国应用生物系统公司）呈阳性的患者（53名女性，47名男性；平均年龄：44.4±10.4岁）纳入研究，这些患者于2002年至2005年间入住切拉尔·巴亚尔大学医学院医院。患者的HCV-RNA定量水平在10⁴-10⁸拷贝/毫升之间。使用Invitek RTP DNA/RNA病毒微量试剂盒从分离出的HCV-RNA中获得的互补DNA，用选定的引物进行基因分型[第一次聚合酶链反应使用引物11（5'-AGG TCT CTG AGA CCG TGC ACC ATG AGC AC-3'）和引物13（5'-CTG TGA GGA ACT ACT GTC TT-3'）；第二次聚合酶链反应使用引物12（5'-ACT GCC TGA TAG GGT GCT TGC GAG TG-3'）和引物14（5'-CAC GCA GAA AGC GTC TAG-3'）]。聚合酶链反应产物用Invisorb Spin PCRapid试剂盒纯化，并在ABI Prism 310基因分析仪中用BigDye Terminator v3.1循环测序试剂盒进行测序。92%的患者（92%）检测出基因型1，7%的患者检测出基因型2和4，1名患者（1%）无法鉴定出HCV基因型。在评估亚型时，90名患者（90%）确定为基因型1b，5名患者（5%）为基因型4a，2名患者（2%）为基因型1a，2名患者（2%）为基因型2a。总之，与之前在土耳其获得的数据一致，1b被发现是马尼萨地区最常见的HCV基因型，其次是基因型4a，尽管较为罕见。这项研究的数据对于HCV感染患者的治疗安排和随访尤其值得关注。
