Stolarczyk Mariusz, Ekiert Radosław, Krzek Jan, Rzeszutko Włodzimierz
Department of Inorganic and Analytical Chemistry, Collegium Medium, Jagiellonian University, Cracow, Poland.
Acta Pol Pharm. 2006 May-Jun;63(3):169-73.
A procedure for simultaneous determination of metoprolol and hydrochlorothiazide in tablets by employing derivative spectrophotometry, "zero-crossing" method was developed. The third order derivative absorption spectra at lambda approximately 281 nm were used for metoprolol and the first order derivative spectra at lambda approximately 282 nm were used for hydrochlorothiazide. No interferences were found between both determined constituents and those of matrix. A good accuracy and precision of simultaneous determination of metoprolol and hydrochlorothiazide were confirmed by statistical analysis. The recovery of individual constituents under established conditions is very high and ranges from 98.79% to 99.39%. Linearity is maintained within a wide concentration range from 100.0 microg mL(-1) to 300.0 microg mL(-1) and from 12.5 microg mL(-1) to 37.5 microg mL(-1) for metoprolol and hydrochlorothiazide, respectively. The detection limit is 5.0 microg mL(-1) for metoprolol and 1.5 microg mL(-1) for hydrochlorothiazide. The corresponding quantitation limits are 15.0 microg mL(-1) for metoprolol and 4.5 microg mL(-1) for hydrochlorothiazide.
建立了一种采用导数分光光度法“零交叉”法同时测定片剂中美托洛尔和氢氯噻嗪的方法。在约281nm波长处的三阶导数吸收光谱用于美托洛尔，在约282nm波长处的一阶导数光谱用于氢氯噻嗪。在测定的两种成分与基质成分之间未发现干扰。通过统计分析证实了同时测定美托洛尔和氢氯噻嗪具有良好的准确度和精密度。在既定条件下，各成分的回收率非常高，范围为98.79%至99.39%。美托洛尔和氢氯噻嗪的线性分别在100.0μg mL(-1)至300.0μg mL(-1)和12.5μg mL(-1)至37.5μg mL(-1)的宽浓度范围内保持。美托洛尔的检测限为5.0μg mL(-1)，氢氯噻嗪的检测限为1.5μg mL(-1)。相应的定量限美托洛尔为15.0μg mL(-1)，氢氯噻嗪为4.5μg mL(-1)。
