Dialectica S.r.l., c/o Nerviano Medical Sciences, V.le Pasteur 10, 20014 Nerviano MI, Italy.
BMC Neurosci. 2010 Jan 19;11:7. doi: 10.1186/1471-2202-11-7.
There is an urgent need of neuronal cell models to be applied to high-throughput screening settings while recapitulating physiological and/or pathological events occurring in the Central Nervous System (CNS). Stem cells offer a great opportunity in this direction since their self renewal capacity allows for large scale expansion. Protocols for directed differentiation also promise to generate populations of biochemically homogenous neuronal progenies. NS (Neural Stem) cells are a novel population of stem cells that undergo symmetric cell division in monolayer and chemically defined media, while remaining highly neurogenic.
We report the full adaptation of the NS cell systems for their growth and neuronal differentiation to 96- and 384-well microplates. This optimized system has also been exploited in homogeneous and high-content assays.
Our results show that these mouse NS cells may be suitable for a series of applications in high-throughput format.
迫切需要神经元细胞模型应用于高通量筛选环境，同时再现中枢神经系统（CNS）中发生的生理和/或病理事件。干细胞为此提供了很好的机会，因为它们的自我更新能力允许大规模扩增。定向分化的方案也有望产生生化同质的神经元祖细胞群体。神经干细胞（NS 细胞）是一种新型的干细胞，它们在单层和化学定义的培养基中进行对称细胞分裂，同时保持高度神经发生能力。
我们报告了 NS 细胞系统在 96 孔和 384 孔微孔板中生长和神经元分化的完全适应。这个优化的系统也被用于同质和高内涵的测定。
我们的结果表明，这些小鼠 NS 细胞可能适合一系列高通量应用。
