冷冻粪便样本可提高实时 PCR 检测难辨内阿米巴和溶组织内阿米巴的效果。
Freezing of stool samples improves real-time PCR detection of Entamoeba dispar and Entamoeba histolytica.
机构信息
Department of Clinical Sciences, Institute of Tropical Medicine (ITM), Antwerp, Belgium.
出版信息
J Microbiol Methods. 2010 Mar;80(3):310-2. doi: 10.1016/j.mimet.2010.01.001. Epub 2010 Jan 18.
PMID:20085792
Abstract
PCR as a tool for intestinal parasite diagnosis is expanding since differentiation between Entamoeba histolytica and Entamoeba dispar cysts is impossible with microscopy. Since pre-analytical factors influence DNA detection, we evaluated with real-time PCR the influence of storage time and temperature. We demonstrated an improved DNA detection in frozen stool samples.
摘要
PCR 作为一种肠道寄生虫诊断工具正在不断发展，因为用显微镜无法区分溶组织内阿米巴和迪斯帕内阿米巴的包囊。由于分析前因素会影响 DNA 的检测，因此我们使用实时 PCR 评估了储存时间和温度的影响。我们证明了冷冻粪便样本中 DNA 检测的改善。
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