Department of Food and Nutrition, Inha University, 253 Yonghyundong, Namku, Incheon 402-751, Republic of Korea.
N Biotechnol. 2010 Sep 30;27(4):435-9. doi: 10.1016/j.nbt.2010.01.003. Epub 2010 Jan 18.
This study investigated the stability of meoru (wild vine grape) anthocyanins in the aqueous solution under singlet oxygen. Freeze-dried meoru (1 kg) contained 179.98 mg anthocyanins including delphinidin-3-glucoside, malvidin-3,5-diglucoside, cyanidin-3,5-diglucoside, malvidin-3-glucoside, and cyanidin-3-glucoside. Malvidin-3,5-diglucoside and cyanidin-3-glucoside were the meoru anthocyanins at the highest and the lowest concentration, respectively. Little decrease in total anthocyanins in the aqueous solution was observed in the dark with or without riboflavin, or with light without riboflavin. Singlet oxygen degraded the meoru anthocyanins in the aqueous solution, which suggested chemical quenching of singlet oxygen by the anthocyanins. Degradation of the meoru anthocyanins was structure-dependent; diglucoside anthocyanins were more stable than monoglucoside. And malvidin glucoside was more stable than delphinidin or cyanidin glucoside, which suggested the number of hydroxy groups in the structure was partly related with the anthocyanin stability under singlet oxygen. This is the first report on anthocyanins stability affected by its structure under singlet oxygen.
本研究考察了在单线态氧下水溶液中野葡萄花色苷的稳定性。冻干的野葡萄（1 公斤）含有 179.98 毫克花色苷，包括矢车菊素-3-葡萄糖苷、二甲酸锦葵素-3,5-二葡萄糖苷、矢车菊素-3,5-二葡萄糖苷、二甲酸锦葵素-3-葡萄糖苷和矢车菊素-3-葡萄糖苷。二甲酸锦葵素-3,5-二葡萄糖苷和矢车菊素-3-葡萄糖苷是野葡萄花色苷中浓度最高和最低的两种。在黑暗中，无论是否有核黄素，或在没有核黄素的光线下，水溶液中的总花色苷含量几乎没有下降。单线态氧降解了水溶液中的野葡萄花色苷，这表明花色苷通过化学猝灭单线态氧。野葡萄花色苷的降解与结构有关；二糖苷花色苷比单糖苷更稳定。锦葵素糖苷比矢车菊素或飞燕草素糖苷更稳定，这表明结构中羟基的数量与花色苷在单线态氧下的稳定性部分相关。这是第一个关于花色苷在单线态氧下受其结构影响的稳定性的报告。
