Department of Thoracic and Cardiovascular Surgery, Nara Medical University, Kashihara, Nara, Japan.
Jpn J Clin Oncol. 2010 Apr;40(4):319-26. doi: 10.1093/jjco/hyp181. Epub 2010 Jan 18.
Prostate stem cell antigen was originally identified as an overexpressed gene in prostate cancer and its overexpression correlated with disease progression and prognosis. In this study, we investigated the clinical significance and therapeutic potential of prostate stem cell antigen expression in non-small cell lung cancer.
Prostate stem cell antigen expression was examined by immunohistochemistry in 97 primary tumors and 21 metastatic lymph nodes from non-small cell lung cancer patients who underwent curative resection from January 2001 through March 2003. Therapeutic potential of targeting prostate stem cell antigen was further examined by small interfering RNA method using human lung cancer cell line (A549).
Prostate stem cell antigen protein expression was detected in 94 of 97 primary lesions (97%) and all metastatic lymph nodes. Prostate stem cell antigen expression intensity was positively correlated with advanced pathological T-factor and stage (T1 vs. T2-4, P = 0.014; Stage I vs. Stages II-IV, P = 0.029, respectively). The prognosis of patients with low prostate stem cell antigen expression was significantly better than those with high prostate stem cell antigen expression (5-year disease-free survival rate; 90% vs. 53%, P = 0.001). Finally, small interfering RNA-mediated knockdown of prostate stem cell antigen resulted in the inhibition of lung cancer cell growth.
Prostate stem cell antigen is highly expressed in non-small cell lung cancer and may be functionally important for this fatal disease.
前列腺干细胞抗原最初被鉴定为前列腺癌中过表达的基因，其过表达与疾病进展和预后相关。本研究探讨了前列腺干细胞抗原在非小细胞肺癌中的表达的临床意义和治疗潜力。
采用免疫组织化学方法检测 2001 年 1 月至 2003 年 3 月期间接受根治性切除术的 97 例非小细胞肺癌患者的 97 例原发肿瘤和 21 例转移性淋巴结中前列腺干细胞抗原的表达。采用小干扰 RNA 法（人肺癌细胞系 A549）进一步研究靶向前列腺干细胞抗原的治疗潜力。
97 例原发灶（97%）和所有转移淋巴结均检测到前列腺干细胞抗原蛋白表达。前列腺干细胞抗原表达强度与晚期病理 T 因子和分期呈正相关（T1 与 T2-4，P = 0.014；I 期与 II-IV 期，P = 0.029）。低前列腺干细胞抗原表达患者的预后明显好于高前列腺干细胞抗原表达患者（5 年无病生存率；90%对 53%，P = 0.001）。最后，小干扰 RNA 介导的前列腺干细胞抗原敲低导致肺癌细胞生长受到抑制。
前列腺干细胞抗原在非小细胞肺癌中高度表达，可能对这种致命疾病具有重要的功能作用。
