Northwest A&F University, Yangling, Shaanxi, China.
Biochem Genet. 2010 Jun;48(5-6):465-71. doi: 10.1007/s10528-009-9329-0. Epub 2010 Jan 20.
Peroxisome proliferator-activated receptor alpha (PPARA) is involved in fatty acid oxidation by upregulating the expression of acyl-coenzyme A oxidase and carnitine palmitoyltransferase. In this study, PPARA gene variations in four chicken breeds (Guyuan, Wenchang, Tibetan, and Hisex) were detected by PCR-SSCP and DNA sequencing. The results indicated six genotypes (AA-EF). When compared with the PPARA reference sequence (GenBank accession no. AF163809), the nucleotide sequences of genotypes AA, BB, AB, and CC revealed silent mutations in the three Chinese breeds. The nucleotide sequences of genotypes DD and EF in Hisex showed several frame-shift mutations, implying variations involving five alleles of the PPARA gene in chicken breeds. In addition, the distribution of genotype frequency within the PPARA gene was significantly different in the four breeds studied, implying that this locus would probably be an effective marker in marker-assisted selection for layer, meat-and-egg, and broiler breeds.
过氧化物酶体增殖物激活受体 α（PPARA）通过上调酰基辅酶 A 氧化酶和肉碱棕榈酰基转移酶的表达来参与脂肪酸氧化。在这项研究中，通过 PCR-SSCP 和 DNA 测序检测了四个鸡品种（固原、文昌、藏鸡和海赛克斯）中的 PPARA 基因变异。结果表明存在六种基因型（AA-EF）。与 PPARA 参考序列（GenBank 登录号 AF163809）相比，基因型 AA、BB、AB 和 CC 的核苷酸序列在三个中国品种中显示出沉默突变。Hisex 中基因型 DD 和 EF 的核苷酸序列显示出几个移码突变，这意味着鸡品种中 PPARA 基因的五个等位基因发生了变异。此外，PPARA 基因内基因型频率的分布在四个研究品种中存在显著差异，这表明该基因座可能是蛋鸡、肉蛋兼用鸡和肉鸡品种的标记辅助选择的有效标记。
