Department of Genetics and Microbiology, M. Curie-Skłodowska University, Akademicka 19 st., Lublin, Poland.
Antonie Van Leeuwenhoek. 2010 May;97(4):351-61. doi: 10.1007/s10482-010-9414-8. Epub 2010 Jan 20.
Robinia pseudoacacia microsymbionts from plants growing in Poland and Japan were evaluated for phylogeny and taxonomic position by genomic approach. Based on the comparative analyses of atpD (368 bp) and dnaK (573 bp) gene sequences as well as 16S rDNA restriction analysis (RFLP-16S rDNA), R. pseudoacacia microsymbionts were identified as Mesorhizobium strains. In dnaK and atpD gene phylograms R. pseudoacacia nodulators formed robust, monophyletic clusters with Mesorhizobium species with the nucleotide sequence similarity of 91-98% and 90-98%, respectively. The classification of R. pseudoacacia rhizobia to the genus Mesorhizobium was also supported by amplified 16S rDNA restriction analysis. The studied bacteria formed common clusters with Mesorhizobium species, and their DNA patterns were identical or nearly identical to Mesorhizobium genus strains. When DNA-DNA hybridization was performed, the total DNA of the representative R. pseudoacacia rhizobia exhibited 51-75% relatedness to DNA of Mesorhizobium amorphae ICMP15022 strain and below 41% to DNA of other Mesorhizobium species. These results showed that R. pseudoacacia and M. amorphae belong to the same genomospecies. The G+C content of DNA of R. pseudoacacia two microsymbionts was 59.7 and 60.6 mol% compared to 61-64 mol% across M. amorphae strains.
从生长在波兰和日本的刺槐共生体中评估了罗宾逊氏菌的系统发育和分类地位，采用基因组方法。基于atpD（368 bp）和dnaK（573 bp）基因序列以及 16S rDNA 限制分析（RFLP-16S rDNA）的比较分析，将刺槐共生体鉴定为中慢生根瘤菌菌株。在 dnaK 和 atpD 基因系统发育树中，刺槐根瘤菌形成了与中慢生根瘤菌物种具有强壮、单系聚类的簇，核苷酸序列相似性分别为 91-98%和 90-98%。扩增的 16S rDNA 限制分析也支持将刺槐根瘤菌分类为中慢生根瘤菌属。研究的细菌与中慢生根瘤菌物种形成了共同的聚类，它们的 DNA 模式与中慢生根瘤菌属菌株相同或几乎相同。当进行 DNA-DNA 杂交时，代表性刺槐根瘤菌的总 DNA 与中慢生根瘤菌 M. amorphae ICMP15022 菌株的 DNA 表现出 51-75%的相关性，而与其他中慢生根瘤菌物种的 DNA 相关性低于 41%。这些结果表明，刺槐和 M. amorphae 属于同一基因组种。与中慢生根瘤菌菌株的 61-64 mol%相比，刺槐两个共生体的 DNA 的 G+C 含量分别为 59.7 和 60.6 mol%。
