Department of Biology, Istanbul University, Vezneciler, Turkey.
Cell Biochem Funct. 2010 Mar;28(2):107-13. doi: 10.1002/cbf.1625.
The protective effects of an antioxidant combination in kidney injury induced by the injection of D-galactosamine (D-GaIN) were examined in the present study. Sprague Dawley female rats were used and divided into four groups as follows: (1) animals injected physiological saline solution, intraperitoneally, (2) animals treated with the combination of ascorbic acid (100 mg kg(-1) day(-1)), beta-carotene (15 mg kg(-1) day(-1)), alpha-tocopherol (100 mg kg(-1) day(-1)), and sodium selenate (0.2 mg kg(-1) day(-1)) for three days orally, (3) rats injected D-GaIN (500 mg kg(-1)) intraperitoneally as a single dose, and (4) animals treated with the antioxidant combination for three days, then injected D-GaIN. The tissue and blood samples of animals were collected for morphological and biochemical evaluations. Histopathological injury in kidney tissues was observed together with a significant increase in tissue lipid peroxidation (LPO) level, myeloperoxidase (MPO), lactate dehydrogenase, catalase and superoxide dismutase (SOD) activities, and serum creatinine and urea levels, and a significant decrease in glutathione level and glutathione peroxidase activity in D-GaIN injected rats. However, a decrease in the degenerative changes was detected in the kidney tissue of D-GaIN + antioxidant group, and biochemical results showed reversed effects. In conclusion, it seems reasonable to conclude that the treatment of the antioxidant combination has a protective effect on D-GaIN-induced kidney injury of rats.
本研究旨在探讨抗氧化剂混合物对注射半乳糖胺（D-GaIN）引起的肾损伤的保护作用。使用 Sprague Dawley 雌性大鼠，并分为以下四组：（1）腹腔注射生理盐水的动物；（2）用抗坏血酸（100 mg kg(-1) day(-1)）、β-胡萝卜素（15 mg kg(-1) day(-1)）、α-生育酚（100 mg kg(-1) day(-1)）和亚硒酸钠（0.2 mg kg(-1) day(-1)）组成的混合物处理的动物，每天口服一次，持续三天；（3）腹腔注射 D-GaIN（500 mg kg(-1)）的大鼠，单次注射；（4）用抗氧化剂混合物处理三天，然后注射 D-GaIN 的动物。收集动物的组织和血液样本进行形态学和生化评估。观察到肾组织的组织学损伤，同时组织脂质过氧化（LPO）水平、髓过氧化物酶（MPO）、乳酸脱氢酶、过氧化氢酶和超氧化物歧化酶（SOD）活性、血清肌酐和尿素水平显著升高，谷胱甘肽水平和谷胱甘肽过氧化物酶活性显著降低在注射 D-GaIN 的大鼠中。然而，在 D-GaIN +抗氧化剂组的肾组织中检测到退行性变化减少，生化结果显示出相反的效果。总之，似乎可以合理地得出结论，抗氧化剂混合物的治疗对 D-GaIN 诱导的大鼠肾损伤具有保护作用。
