血小板参数 delta 值在 ADVIA 120 上对储存血小板功能反应性的有用性。
Usefulness of delta value of platelet parameters on ADVIA 120 for the functional reactivity of stored platelets.
机构信息
Department of Laboratory Medicine, Ajou University School of Medicine, Suwon, South Korea.
出版信息
J Clin Lab Anal. 2010;24(1):38-43. doi: 10.1002/jcla.20364.
An agonist-induced expression of CD62P by flow cytometry analysis for evaluating platelet functional reactivity has some disadvantages. We investigated the usefulness of platelet parameters by ADVIA 120 to predict an agonist-induced expression of CD62P in stored platelets. The CD62P expression by flow cytometry and the platelet parameters by ADVIA 120 were studied in samples from 27 platelet pheresis products. Delta (Delta) values were calculated as the degree of change of the platelet parameters studied with or without adenosine 5'-diphosphate sodium (ADP) stimulation. The CD62P expression of the ADP-activated platelets were correlated with the Delta platelet count (r=0.517) in the short-term storage group (within 10 hr from preparation), with the platelet component distribution width (PCDW) without ADP (r=-0.744) and the DeltaPCDW (r=-0.755) in the long-term storage group (after 10 hr from preparation). Therefore, the delta values of platelet parameters on ADVIA 120 analysis in platelets between with and without ADP stimulation could be useful as a simple predictor for the functional reactivity of stored platelets.
通过流式细胞术分析诱导 CD62P 的表达来评估血小板功能反应性有一些缺点。我们研究了 ADVIA 120 血小板参数在预测储存血小板诱导 CD62P 表达中的作用。对 27 份血小板单采产品的样本进行了流式细胞术检测 CD62P 表达和 ADVIA 120 血小板参数的研究。计算了血小板参数在有无二磷酸腺苷（ADP）刺激下的变化程度（Delta）值。ADP 激活血小板的 CD62P 表达与短期储存组（制备后 10 小时内）的血小板计数 Delta 值（r=0.517）、无 ADP 时的血小板分布宽度（PCDW）（r=-0.744）和长期储存组（制备后 10 小时后）的 DeltaPCDW（r=-0.755）相关。因此，ADP 刺激前后 ADVIA 120 分析中血小板参数的 Delta 值可以作为储存血小板功能反应性的简单预测指标。
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