[由转录共激活因子SAYP形成的新型复合物]

[Novel complex, formed by transcription coactivator SAYP].

作者信息

Soshnikova N V, Vorob'eva N E, Krasnov A N, Georgieva S G, Nabirochkina E N, Shidlovskiĭ Iu V

出版信息

Mol Biol (Mosk). 2009 Nov-Dec;43(6):1055-62.

PMID:20088382

Abstract

The multisubunit complex which contains the novel evolutionarily conservative transcription factor SAYP was isolated and the protein composition of the complex was determined. It was shown that SAYP unites two complexes with different functions in transcription activation: the chromatin - remodeling complex PBAP (SWI/SNF) and the main component of preinitiation complex of Pol II, general transcription factor TFIID. The isolated super-complex contained the full set of PBAP and TFIID subunits. All components of supercomplex (SAYP, TFIID and PBAP) are essential for its effective interaction with promoters of SAYP-dependent genes.

摘要

包含新型进化保守转录因子SAYP的多亚基复合物被分离出来，并确定了该复合物的蛋白质组成。结果表明，SAYP在转录激活中结合了两种具有不同功能的复合物：染色质重塑复合物PBAP（SWI/SNF）和Pol II起始前复合物的主要成分、通用转录因子TFIID。分离出的超级复合物包含PBAP和TFIID亚基的全套。超级复合物的所有成分（SAYP、TFIID和PBAP）对于其与SAYP依赖性基因启动子的有效相互作用至关重要。